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Sample GSM1528628 Query DataSets for GSM1528628
Status Public on Sep 29, 2017
Title Sample 8 - hlk - Z27
Sample type RNA
 
Source name FACS purified GFP+ T-lymphoblasts
Organism Danio rerio
Characteristics model: hlk sample
cell type: Sorted GFP+ T-lymphoblasts
Treatment protocol FACS purified GFP+ T-lymphoblasts from the bodies of hMYC-ER and hlk zebrafish models at the final stage of disease
Extracted molecule total RNA
Extraction protocol Total RNA extraction using Trizol protocol
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix).
 
Hybridization protocol Following fragmentation, 12,5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Zebrafish Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450DX.
Scan protocol GeneChips were scanned using the Scan GCS3000.
Description Gene expression data from FACS purified GFP+ T-lymphoblasts of hlk specimen at the final stage of disease
Data processing The data were normalized using R software [www.r-ptoject.org] with BioConductor package [www.bioconductor.org]
 
Submission date Oct 21, 2014
Last update date Sep 29, 2017
Contact name Chiara Borga
E-mail(s) chiaraborga@gmail.com
Organization name University of Padua
Street address Corso Stati Uniti,4
City Padova
ZIP/Postal code 35127
Country Italy
 
Platform ID GPL1319
Series (1)
GSE62541 Discovery of Central Nervous System Infiltration in Zebrafish T Cell Acute Lymphoblastic Leukemia

Data table header descriptions
ID_REF
VALUE MAS5
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 475.642 P 0.000972149
AFFX-BioB-M_at 640.435 P 5.16732e-05
AFFX-BioB-3_at 355.001 P 0.00110197
AFFX-BioC-5_at 1030.44 P 5.16732e-05
AFFX-BioC-3_at 1678.57 P 0.000224668
AFFX-BioDn-5_at 2337.92 P 4.42873e-05
AFFX-BioDn-3_at 5689.99 P 7.00668e-05
AFFX-CreX-5_at 17966.6 P 4.42873e-05
AFFX-CreX-3_at 31754 P 4.42873e-05
AFFX-DapX-5_at 77.1883 M 0.0584438
AFFX-DapX-M_at 307.99 P 0.0032123
AFFX-DapX-3_at 529.95 P 0.000195116
AFFX-LysX-5_at 5.57933 A 0.852082
AFFX-LysX-M_at 11.3344 A 0.883887
AFFX-LysX-3_at 65.8111 A 0.15671
AFFX-PheX-5_at 61.6232 A 0.227636
AFFX-PheX-M_at 55.4612 A 0.287743
AFFX-PheX-3_at 131.424 P 0.0125468
AFFX-ThrX-5_at 44.6501 A 0.749204
AFFX-ThrX-M_at 110.138 A 0.083592

Total number of rows: 15617

Table truncated, full table size 561 Kbytes.




Supplementary file Size Download File type/resource
GSM1528628_Z27_Zebrafish_.CEL.gz 1.8 Mb (ftp)(http) CEL
GSM1528628_Z27_Zebrafish_.mas5.CHP.gz 176.8 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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