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Sample GSM1529488 Query DataSets for GSM1529488
Status Public on Oct 31, 2014
Title Chimpanzee iPS cell clone 402
Sample type RNA
 
Source name Chimpanzee induced pluripotent stem cell
Organism Pan troglodytes
Characteristics cell type: iPS cell
Treatment protocol Conventional human ES cell cuture method.
Growth protocol Human and Chimpanzee iPS cells were maintained on MEFs in a medium containing DMEM/F12, 100mM L-glutamine/2-mercaptoethanol, 20% knockout serum replacement, 1X non-essential aminoacids and 5ng/ml human FGF2. Human dermal fibroblast were maintained in a medium containing DMEM, 10% fetal bovine serum.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from each cell subset using Sepasol-RNA 1 Super G (nacalai) according to the manufacturer's protocol.
Label biotin
Label protocol RNA was amplified, labeled and fragmented using GeneChip® 3' IVT Express Kit (affymetrix) according to the manufacturer's protocol.
 
Hybridization protocol Gene chips were hybridized with biotinylated cRNA according to the manufacturer's protocol (Affymetrix). Chips were washed and stained with GeneChip® Fluidics Station 450.
Scan protocol Scanned with Affymetrix GeneChip®Scanner 3000 7G.
Data processing Raw CEL files were normalized using the Robust Multichip Average (RMA) method implemented in Genespring GX 12.5 (Agilent).
 
Submission date Oct 22, 2014
Last update date Oct 31, 2014
Contact name Makoto Hamasaki
E-mail(s) makotohmsk@gmail.com
Organization name Kumamoto univ
Street address honjo 2-2-1
City kumamoto
ZIP/Postal code 860-0812
Country Japan
 
Platform ID GPL570
Series (1)
GSE62572 Global gene expression profiling human and Chimpanzee induced pluripotent stem (iPS) cell

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
AFFX-BioB-5_at 7.464859
AFFX-BioB-M_at 7.615595
AFFX-BioB-3_at 7.5204334
AFFX-BioC-5_at 8.366845
AFFX-BioC-3_at 9.175045
AFFX-BioDn-5_at 10.075569
AFFX-BioDn-3_at 11.225139
AFFX-CreX-5_at 12.215874
AFFX-CreX-3_at 12.785867
AFFX-DapX-5_at 5.3554115
AFFX-DapX-M_at 8.296051
AFFX-DapX-3_at 9.712789
AFFX-LysX-5_at 4.816102
AFFX-LysX-M_at 5.4378986
AFFX-LysX-3_at 6.695693
AFFX-PheX-5_at 4.7980747
AFFX-PheX-M_at 5.8806376
AFFX-PheX-3_at 6.2899237
AFFX-ThrX-5_at 4.2455544
AFFX-ThrX-M_at 5.2718673

Total number of rows: 54675

Table truncated, full table size 1087 Kbytes.




Supplementary file Size Download File type/resource
GSM1529488_C402_iPSC.CEL.gz 3.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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