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Sample GSM1529490 Query DataSets for GSM1529490
Status Public on Oct 31, 2014
Title Human iPS cell derived from N3 fibroblast clone 2
Sample type RNA
 
Source name Human induced pluripotent stem cell
Organism Homo sapiens
Characteristics cell type: iPS cell
Treatment protocol Conventional human ES cell cuture method.
Growth protocol Human and Chimpanzee iPS cells were maintained on MEFs in a medium containing DMEM/F12, 100mM L-glutamine/2-mercaptoethanol, 20% knockout serum replacement, 1X non-essential aminoacids and 5ng/ml human FGF2. Human dermal fibroblast were maintained in a medium containing DMEM, 10% fetal bovine serum.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from each cell subset using Sepasol-RNA 1 Super G (nacalai) according to the manufacturer's protocol.
Label biotin
Label protocol RNA was amplified, labeled and fragmented using GeneChip® 3' IVT Express Kit (affymetrix) according to the manufacturer's protocol.
 
Hybridization protocol Gene chips were hybridized with biotinylated cRNA according to the manufacturer's protocol (Affymetrix). Chips were washed and stained with GeneChip® Fluidics Station 450.
Scan protocol Scanned with Affymetrix GeneChip®Scanner 3000 7G.
Data processing Raw CEL files were normalized using the Robust Multichip Average (RMA) method implemented in Genespring GX 12.5 (Agilent).
 
Submission date Oct 22, 2014
Last update date Oct 31, 2014
Contact name Makoto Hamasaki
E-mail(s) makotohmsk@gmail.com
Organization name Kumamoto univ
Street address honjo 2-2-1
City kumamoto
ZIP/Postal code 860-0812
Country Japan
 
Platform ID GPL570
Series (1)
GSE62572 Global gene expression profiling human and Chimpanzee induced pluripotent stem (iPS) cell

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
AFFX-BioB-5_at 7.3322477
AFFX-BioB-M_at 7.4860854
AFFX-BioB-3_at 7.404196
AFFX-BioC-5_at 8.606257
AFFX-BioC-3_at 9.314681
AFFX-BioDn-5_at 10.2942705
AFFX-BioDn-3_at 11.202628
AFFX-CreX-5_at 12.554919
AFFX-CreX-3_at 13.006778
AFFX-DapX-5_at 8.043707
AFFX-DapX-M_at 8.947171
AFFX-DapX-3_at 9.763973
AFFX-LysX-5_at 4.772199
AFFX-LysX-M_at 5.7150745
AFFX-LysX-3_at 6.2427716
AFFX-PheX-5_at 5.1856546
AFFX-PheX-M_at 5.685101
AFFX-PheX-3_at 6.014644
AFFX-ThrX-5_at 6.135863
AFFX-ThrX-M_at 6.3853025

Total number of rows: 54675

Table truncated, full table size 1087 Kbytes.




Supplementary file Size Download File type/resource
GSM1529490_N3-2_iPSC.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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