|
| Status |
Public on Oct 31, 2014 |
| Title |
Human dermal fibroblast from healthy volunteer 1 |
| Sample type |
RNA |
| |
|
| Source name |
Human dermal fibroblast
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Fibroblast
|
| Treatment protocol |
Conventional human ES cell cuture method.
|
| Growth protocol |
Human and Chimpanzee iPS cells were maintained on MEFs in a medium containing DMEM/F12, 100mM L-glutamine/2-mercaptoethanol, 20% knockout serum replacement, 1X non-essential aminoacids and 5ng/ml human FGF2. Human dermal fibroblast were maintained in a medium containing DMEM, 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from each cell subset using Sepasol-RNA 1 Super G (nacalai) according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
RNA was amplified, labeled and fragmented using GeneChip® 3' IVT Express Kit (affymetrix) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Gene chips were hybridized with biotinylated cRNA according to the manufacturer's protocol (Affymetrix). Chips were washed and stained with GeneChip® Fluidics Station 450.
|
| Scan protocol |
Scanned with Affymetrix GeneChip®Scanner 3000 7G.
|
| Data processing |
Raw CEL files were normalized using the Robust Multichip Average (RMA) method implemented in Genespring GX 12.5 (Agilent).
|
| |
|
| Submission date |
Oct 22, 2014 |
| Last update date |
Oct 31, 2014 |
| Contact name |
Makoto Hamasaki |
| E-mail(s) |
makotohmsk@gmail.com
|
| Organization name |
Kumamoto univ
|
| Street address |
honjo 2-2-1
|
| City |
kumamoto |
| ZIP/Postal code |
860-0812 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE62572 |
Global gene expression profiling human and Chimpanzee induced pluripotent stem (iPS) cell |
|
