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Sample GSM1530108 Query DataSets for GSM1530108
Status Public on Jan 01, 2015
Title female WT, replicate 3 [exon]
Sample type RNA
 
Source name Female MEFs, CAGG-Cre-ER T/+
Organism Mus musculus
Characteristics gender: female
genotype/variation: WT
treatment: vehicle control
Treatment protocol 200nM 4-Hydroxytamoxifen for 4 days. Ethanol represents vehicle control.
Growth protocol DMEM supplemented with 10% FCS, pen/strep
Extracted molecule total RNA
Extraction protocol QIAGEN Rneasy
Label biotin
Label protocol according to manufacturer's instructions
 
Hybridization protocol according to manufacturer's instructions
Scan protocol according to manufacturer's instructions
Data processing We performed exon- (probeset-) and gene-level analysis using the Affymetrix Power Tools (version=1.14.3.1). The rma-sketch algorithm was used to normalize the intensities in CEL files and detection p-values were generated for each probeset using the “detection above background” (DABG) method.
 
Submission date Oct 22, 2014
Last update date Jan 01, 2015
Contact name Thomas Manke
Organization name Max-Planck-Institute for Immunobiology and Epigenetics
Department Bioinformatics and Deep-Sequencing Unit
Street address Stuebeweg 51
City Freiburg im Breisgau
ZIP/Postal code 79108
Country Germany
 
Platform ID GPL10740
Series (2)
GSE62617 MOF regulates cell cycle progression
GSE70707 MOF maintains Transcriptional Programs required to protect cells against Stress
Relations
Alternative to GSM1530056 (gene-level analysis )

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
10338001 10.14005 0
10338002 6.12124 0.33412
10338003 8.3313 0
10338004 7.0472 0
10338005 2.27017 0.94642
10338006 2.5318 0.67915
10338007 2.9622 0.93921
10338008 3.89502 0.81552
10338009 7.91428 0.40463
10338010 2.28691 0.70474
10338011 5.64767 0.62177
10338012 2.39721 0.23757
10338013 2.14182 0.44507
10338014 2.20786 0.76304
10338015 2.18054 0.6385
10338016 7.3237 0.70601
10338017 11.38857 0
10338018 6.58447 0.42491
10338019 5.18995 0.3225
10338020 7.81462 0.98103

Total number of rows: 65533

Table truncated, full table size 1542 Kbytes.




Supplementary file Size Download File type/resource
GSM1530108_d4_36-C.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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