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Sample GSM1530113 Query DataSets for GSM1530113
Status Public on Jan 01, 2015
Title male WT, replicate 2 [exon]
Sample type RNA
 
Source name Male MEFs, CAGG-Cre-ER T/+
Organism Mus musculus
Characteristics gender: male
genotype/variation: WT
treatment: vehicle control
Treatment protocol 200nM 4-Hydroxytamoxifen for 4 days. Ethanol represents vehicle control.
Growth protocol DMEM supplemented with 10% FCS, pen/strep
Extracted molecule total RNA
Extraction protocol QIAGEN Rneasy
Label biotin
Label protocol according to manufacturer's instructions
 
Hybridization protocol according to manufacturer's instructions
Scan protocol according to manufacturer's instructions
Data processing We performed exon- (probeset-) and gene-level analysis using the Affymetrix Power Tools (version=1.14.3.1). The rma-sketch algorithm was used to normalize the intensities in CEL files and detection p-values were generated for each probeset using the “detection above background” (DABG) method.
 
Submission date Oct 22, 2014
Last update date Jan 01, 2015
Contact name Thomas Manke
Organization name Max-Planck-Institute for Immunobiology and Epigenetics
Department Bioinformatics and Deep-Sequencing Unit
Street address Stuebeweg 51
City Freiburg im Breisgau
ZIP/Postal code 79108
Country Germany
 
Platform ID GPL10740
Series (2)
GSE62617 MOF regulates cell cycle progression
GSE70707 MOF maintains Transcriptional Programs required to protect cells against Stress
Relations
Alternative to GSM1530061 (gene-level analysis )

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
10338001 11.00051 0
10338002 6.24486 0.24991
10338003 9.17503 0
10338004 7.94445 0
10338005 2.17372 0.97644
10338006 2.46615 0.92125
10338007 3.00132 0.95612
10338008 3.98069 0.89386
10338009 8.06102 0.36267
10338010 2.19382 0.90289
10338011 5.78775 0.77832
10338012 2.28056 0.8255
10338013 1.97483 0.9399
10338014 2.05199 0.97641
10338015 2.04574 0.85014
10338016 7.46842 0.77318
10338017 12.23789 0
10338018 6.6907 0.45611
10338019 5.37801 0.25692
10338020 7.98951 0.9877

Total number of rows: 65533

Table truncated, full table size 1546 Kbytes.




Supplementary file Size Download File type/resource
GSM1530113_d4_40-B.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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