|
| Status |
Public on Jan 01, 2015 |
| Title |
male KO, replicate 2 [exon] |
| Sample type |
RNA |
| |
|
| Source name |
Male MEFs, CAGG-Cre-ER T/+, 4OHT treated
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
genotype/variation: KO
treatment: 200nM 4-Hydroxytamoxifen for 4 days
|
| Treatment protocol |
200nM 4-Hydroxytamoxifen for 4 days. Ethanol represents vehicle control.
|
| Growth protocol |
DMEM supplemented with 10% FCS, pen/strep
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN Rneasy
|
| Label |
biotin
|
| Label protocol |
according to manufacturer's instructions
|
| |
|
| Hybridization protocol |
according to manufacturer's instructions
|
| Scan protocol |
according to manufacturer's instructions
|
| Data processing |
We performed exon- (probeset-) and gene-level analysis using the Affymetrix Power Tools (version=1.14.3.1). The rma-sketch algorithm was used to normalize the intensities in CEL files and detection p-values were generated for each probeset using the “detection above background” (DABG) method.
|
| |
|
| Submission date |
Oct 22, 2014 |
| Last update date |
Jan 01, 2015 |
| Contact name |
Thomas Manke |
| Organization name |
Max-Planck-Institute for Immunobiology and Epigenetics
|
| Department |
Bioinformatics and Deep-Sequencing Unit
|
| Street address |
Stuebeweg 51
|
| City |
Freiburg im Breisgau |
| ZIP/Postal code |
79108 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10740 |
| Series (2) |
| GSE62617 |
MOF regulates cell cycle progression |
| GSE70707 |
MOF maintains Transcriptional Programs required to protect cells against Stress |
|
| Relations |
| Alternative to |
GSM1530064 (gene-level analysis
) |
