NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM153208 Query DataSets for GSM153208
Status Public on Jan 16, 2007
Title Normal prostate tissue adjacent to tumor from patient No.68 PN68U95B
Sample type RNA
 
Source name PN68, normal prostate tissue adjacent to tumor from patient No.68
Organism Homo sapiens
Characteristics Tissue:normal prostate tissue adjacent to tumor
Treatment protocol Fresh prostate tissues were recovered immediately after surgical removal. Tissues were dissected/trimmed to obtain pure tumor then snap frozen in liquid nitrogen within 30 minutes of excision and stored in -80C until RNA extraction. An H&E stained frozen section of each sample was evaluated by a pathologist, to determine epithelial and stromal content and verify the presence of tumor in the sample. For prostate tissues adjacent to tumor, samples free of cancer cells, high-grade prostatic neoplasia, or any obvious neoplastic alterations, containing at least 60% glandular cells were selected.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Qiagen Rneasy kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U95B array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from normal prostate tissue adjacent to tumor
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200.
 
Submission date Dec 22, 2006
Last update date Jan 16, 2007
Contact name Federico Alberto Monzon
E-mail(s) famonzon@tmhs.org
Organization name The Methodist Hospital
Department Pathology
Lab Molecular Diagnostics
Street address 6565 Fannin St, MS205
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL92
Series (2)
GSE6608 Expression data from Normal Prostate Tissue Adjacent to Tumor
GSE6919 Expression Data from Normal and Prostate Tumor Tissues

Data table header descriptions
ID_REF 12620
VALUE MAS5-calculated Signal intensity

Data table
ID_REF VALUE
41880_at 34.2
41881_at 192.2
41882_at 4.6
41883_at 23.9
41884_at 64.2
41885_at 4.6
41886_r_at 212.9
41887_at 37.1
41888_at 29.6
41889_at 26.3
41890_at 26.2
41891_at 124.5
41892_at 436.2
41893_at 12.1
41894_r_at 38
41895_at 267.3
41896_at 5.5
41897_at 31.6
41898_at 26.3
41899_at 126.8

Total number of rows: 12620

Table truncated, full table size 180 Kbytes.




Supplementary file Size Download File type/resource
GSM153208.CEL.gz 2.4 Mb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap