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Sample GSM153328 Query DataSets for GSM153328
Status Public on Jul 01, 2007
Title wild type placebo 12h, biological rep2
Sample type RNA
 
Source name ventral prostate of wild type FVB mice
Organism Mus musculus
Characteristics Genotype: wild type
Age: 8-10 weeks
Treatment protocol placebo for 12h
Growth protocol standard mouse diet
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol standard procedure carried out at Broad Institute
Scan protocol standard procedure carried out at Broad Institute
Description Gene expression data from ventral prostate of wild type FVB mice and FVB-AKT1 mice treated with placebo or rad001 for 12h, 2d, 6d, 9d, and 15d
Data processing CEL files were normalized with dChip, then expression values were computed and merged and normalized again with dChip.
 
Submission date Dec 22, 2006
Last update date Dec 27, 2006
Contact name qing xu
E-mail(s) qing_xu@dfci.harvard.edu
Phone 6176322930
Organization name dana farber cancer institute
Department Medical Oncology
Lab sellers
Street address 44 binney st
City boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL339
Series (1)
GSE6610 Expression data from murine ventral prostate treated with placebo or RAD001 for 12h, 48h, 6d,9d, and 15d

Data table header descriptions
ID_REF
VALUE dChip normalized and computed expression index

Data table
ID_REF VALUE
1415670_at 264.522
1415671_at 673.6
1415672_at 495.697
1415673_at 41.8773
1415674_a_at 260.134
1415675_at 655.708
1415676_a_at 574.321
1415677_at 123.267
1415678_at 283.152
1415679_at 1188.87
1415680_at 366.052
1415681_at 300.799
1415682_at 222.971
1415683_at 406.031
1415684_at 193.609
1415685_at 205.06
1415686_at 513.375
1415687_a_at 2443.9
1415688_at 643.841
1415689_s_at 214.424

Total number of rows: 22626

Table truncated, full table size 430 Kbytes.




Supplementary file Size Download File type/resource
GSM153328.CEL.gz 3.5 Mb (ftp)(http) CEL

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