|
| Status |
Public on Jul 01, 2007 |
| Title |
placebo 12h, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
ventral prostate of transgenic FVB mice
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: transgenic
Age: 8-10 weeks
|
| Treatment protocol |
placebo for 12h
|
| Growth protocol |
standard mouse diet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
standard procedure carried out at Broad Institute
|
| Scan protocol |
standard procedure carried out at Broad Institute
|
| Description |
Gene expression data from ventral prostate of wild type FVB mice and FVB-AKT1 mice treated with placebo or rad001 for 12h, 2d, 6d, 9d, and 15d
|
| Data processing |
CEL files were normalized with dChip, then expression values were computed and merged and normalized again with dChip.
|
| |
|
| Submission date |
Dec 22, 2006 |
| Last update date |
Dec 27, 2006 |
| Contact name |
qing xu |
| E-mail(s) |
qing_xu@dfci.harvard.edu
|
| Phone |
6176322930
|
| Organization name |
dana farber cancer institute
|
| Department |
Medical Oncology
|
| Lab |
sellers
|
| Street address |
44 binney st
|
| City |
boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE6610 |
Expression data from murine ventral prostate treated with placebo or RAD001 for 12h, 48h, 6d,9d, and 15d |
|
