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Status |
Public on Jul 01, 2007 |
Title |
placebo 15d, biological rep1 |
Sample type |
RNA |
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|
Source name |
ventral prostate of transgenic FVB mice
|
Organism |
Mus musculus |
Characteristics |
Genotype: transgenic Age: 8-10 weeks
|
Treatment protocol |
placebo for 15d
|
Growth protocol |
standard mouse diet
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
|
Hybridization protocol |
standard procedure carried out at Broad Institute
|
Scan protocol |
standard procedure carried out at Broad Institute
|
Description |
Gene expression data from ventral prostate of wild type FVB mice and FVB-AKT1 mice treated with placebo or rad001 for 12h, 2d, 6d, 9d, and 15d
|
Data processing |
CEL files were normalized with dChip, then expression values were computed and merged and normalized again with dChip.
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|
|
Submission date |
Dec 22, 2006 |
Last update date |
Dec 27, 2006 |
Contact name |
qing xu |
E-mail(s) |
qing_xu@dfci.harvard.edu
|
Phone |
6176322930
|
Organization name |
dana farber cancer institute
|
Department |
Medical Oncology
|
Lab |
sellers
|
Street address |
44 binney st
|
City |
boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL339 |
Series (1) |
GSE6610 |
Expression data from murine ventral prostate treated with placebo or RAD001 for 12h, 48h, 6d,9d, and 15d |
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