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Sample GSM1536990 Query DataSets for GSM1536990
Status Public on Jun 17, 2015
Title DMSO_Rep1
Sample type RNA
Source name DMSO treated ARPE-19 cells
Organism Homo sapiens
Characteristics cell line: ARPE-19
agent: DMSO
Treatment protocol ARPE-19 cells were treated with SRPIN803 (10 µM) or the control (0.1% DMSO) for 4 hours.
Growth protocol ARPE-19 cells were maintained at 37 ºC in a 5% CO2-air incubator with Dulbecco's modified Eagle's medium (DMEM)/Ham's F-12 supplemented with 10% fetal bovine serum and 1% Penicillin‐Streptomycin Mixed Solution
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Sepasol®-RNA I Super G (nacalai tesque) according to the manufacturer's instruction. The Isolated total RNA was treated with RQ1 DNase (QIAGEN) and was purified with Sepasol®-RNA I Super G again.
Label biotin
Label protocol Biotinylated aRNA were prepared from 100 ng total RNA using GeneChip® 3' IVT Express Kit (Affymetrix) from 100 ng total RNA according to the manufacturer's instruction
Hybridization protocol Following fragmentation, 12 ug of aRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained using GeneChip Hybridization Wash and Stain Kit in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip Scanner 3000G
Description Gene expression data from DMSO treated ARPE-19 cells
Data processing The data were analyzed with RMA using Agilent GeneSpringGX 12.6.1 software.
Submission date Nov 04, 2014
Last update date Jun 17, 2015
Contact name Masatsugu Denawa
Organization name Kyoto University
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
ZIP/Postal code 6068501
Country Japan
Platform ID GPL570
Series (1)
GSE62947 Expression Data from DMSO or SRPIN803 treated ARPE-19 cells

Data table header descriptions
VALUE log2 RMA signal

Data table
AFFX-BioB-5_at 8.605058
AFFX-BioB-M_at 8.891404
AFFX-BioB-3_at 8.708109
AFFX-BioC-5_at 9.684519
AFFX-BioC-3_at 10.56563
AFFX-BioDn-5_at 11.068687
AFFX-BioDn-3_at 12.4030485
AFFX-CreX-5_at 13.444929
AFFX-CreX-3_at 13.763078
AFFX-DapX-5_at 8.779344
AFFX-DapX-M_at 10.509165
AFFX-DapX-3_at 11.5481205
AFFX-LysX-5_at 6.981387
AFFX-LysX-M_at 7.411062
AFFX-LysX-3_at 8.092575
AFFX-PheX-5_at 7.646641
AFFX-PheX-M_at 8.014857
AFFX-PheX-3_at 8.148474
AFFX-ThrX-5_at 7.162145
AFFX-ThrX-M_at 8.2120695

Total number of rows: 54675

Table truncated, full table size 1085 Kbytes.

Supplementary file Size Download File type/resource
GSM1536990_DMSO_Rep1.CEL.gz 4.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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