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Sample GSM1536992 Query DataSets for GSM1536992
Status Public on Jun 17, 2015
Title DMSO_Rep3
Sample type RNA
 
Source name DMSO treated ARPE-19 cells
Organism Homo sapiens
Characteristics cell line: ARPE-19
agent: DMSO
Treatment protocol ARPE-19 cells were treated with SRPIN803 (10 µM) or the control (0.1% DMSO) for 4 hours.
Growth protocol ARPE-19 cells were maintained at 37 ºC in a 5% CO2-air incubator with Dulbecco's modified Eagle's medium (DMEM)/Ham's F-12 supplemented with 10% fetal bovine serum and 1% Penicillin‐Streptomycin Mixed Solution
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Sepasol®-RNA I Super G (nacalai tesque) according to the manufacturer's instruction. The Isolated total RNA was treated with RQ1 DNase (QIAGEN) and was purified with Sepasol®-RNA I Super G again.
Label biotin
Label protocol Biotinylated aRNA were prepared from 100 ng total RNA using GeneChip® 3' IVT Express Kit (Affymetrix) from 100 ng total RNA according to the manufacturer's instruction
 
Hybridization protocol Following fragmentation, 12 ug of aRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained using GeneChip Hybridization Wash and Stain Kit in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip Scanner 3000G
Description Gene expression data from DMSO treated ARPE-19 cells
Data processing The data were analyzed with RMA using Agilent GeneSpringGX 12.6.1 software.
 
Submission date Nov 04, 2014
Last update date Jun 17, 2015
Contact name Masatsugu Denawa
Organization name Kyoto University
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
ZIP/Postal code 6068501
Country Japan
 
Platform ID GPL570
Series (1)
GSE62947 Expression Data from DMSO or SRPIN803 treated ARPE-19 cells

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.374892
AFFX-BioB-M_at 8.609841
AFFX-BioB-3_at 8.394821
AFFX-BioC-5_at 9.422887
AFFX-BioC-3_at 10.305904
AFFX-BioDn-5_at 10.832462
AFFX-BioDn-3_at 12.2206
AFFX-CreX-5_at 13.279608
AFFX-CreX-3_at 13.629946
AFFX-DapX-5_at 8.903906
AFFX-DapX-M_at 10.454242
AFFX-DapX-3_at 11.497897
AFFX-LysX-5_at 6.9233737
AFFX-LysX-M_at 7.37483
AFFX-LysX-3_at 8.012598
AFFX-PheX-5_at 7.517135
AFFX-PheX-M_at 7.7699714
AFFX-PheX-3_at 8.076084
AFFX-ThrX-5_at 7.4781227
AFFX-ThrX-M_at 8.1820345

Total number of rows: 54675

Table truncated, full table size 1085 Kbytes.




Supplementary file Size Download File type/resource
GSM1536992_DMSO_Rep3.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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