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Sample GSM1536995 Query DataSets for GSM1536995
Status Public on Jun 17, 2015
Title SRPIN803_Rep3
Sample type RNA
 
Source name SRPIN803 treated ARPE-19 cells
Organism Homo sapiens
Characteristics cell line: ARPE-19
agent: SRPIN803 (10 µM)
Treatment protocol ARPE-19 cells were treated with SRPIN803 (10 µM) or the control (0.1% DMSO) for 4 hours.
Growth protocol ARPE-19 cells were maintained at 37 ºC in a 5% CO2-air incubator with Dulbecco's modified Eagle's medium (DMEM)/Ham's F-12 supplemented with 10% fetal bovine serum and 1% Penicillin‐Streptomycin Mixed Solution
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Sepasol®-RNA I Super G (nacalai tesque) according to the manufacturer's instruction. The Isolated total RNA was treated with RQ1 DNase (QIAGEN) and was purified with Sepasol®-RNA I Super G again.
Label biotin
Label protocol Biotinylated aRNA were prepared from 100 ng total RNA using GeneChip® 3' IVT Express Kit (Affymetrix) from 100 ng total RNA according to the manufacturer's instruction
 
Hybridization protocol Following fragmentation, 12 ug of aRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained using GeneChip Hybridization Wash and Stain Kit in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip Scanner 3000G
Description Gene expression data from SRPIN803 treated ARPE-19 cells
Data processing The data were analyzed with RMA using Agilent GeneSpringGX 12.6.1 software.
 
Submission date Nov 04, 2014
Last update date Jun 17, 2015
Contact name Masatsugu Denawa
Organization name Kyoto University
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
ZIP/Postal code 6068501
Country Japan
 
Platform ID GPL570
Series (1)
GSE62947 Expression Data from DMSO or SRPIN803 treated ARPE-19 cells

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.22047
AFFX-BioB-M_at 8.536223
AFFX-BioB-3_at 8.313035
AFFX-BioC-5_at 9.360896
AFFX-BioC-3_at 10.272676
AFFX-BioDn-5_at 10.704348
AFFX-BioDn-3_at 12.238427
AFFX-CreX-5_at 13.242973
AFFX-CreX-3_at 13.621599
AFFX-DapX-5_at 8.499342
AFFX-DapX-M_at 10.265015
AFFX-DapX-3_at 11.421878
AFFX-LysX-5_at 6.5884805
AFFX-LysX-M_at 7.300264
AFFX-LysX-3_at 7.9680796
AFFX-PheX-5_at 7.27927
AFFX-PheX-M_at 7.8037896
AFFX-PheX-3_at 8.055805
AFFX-ThrX-5_at 6.9273806
AFFX-ThrX-M_at 7.7988567

Total number of rows: 54675

Table truncated, full table size 1085 Kbytes.




Supplementary file Size Download File type/resource
GSM1536995_SRPIN803_Rep3.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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