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Status |
Public on Sep 28, 2007 |
Title |
Lung WT mice 12 weeks after MCA/BHT treatment |
Sample type |
RNA |
|
|
Source name |
left lung lobes from three mice pooled
|
Organism |
Mus musculus |
Characteristics |
Strain: FVB/N Gender: male Tissue: lung
|
Treatment protocol |
Wild type FVB/N mice were injected intraperitoneally (i.p.) with one dose of 3-methylcholanthrene (MCA; 15 µg/g mouse weight), followed by six weekly i.p. injections of butylated hydroxytoluene (BHT; the first dose was 150 µg/g mouse weight, and subsequent doses were 200 µg/g mouse weight)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using RNA STAT-60 Reagent (TEL-TEST, INC., Friendswood, TX) with modifications to remove DNA using the Qiagen RNAeasy columns and DNaseI Kit (Qiagen, Valencia, CA). RNA was stored at –70C in RNase-free H2O. Quality and quantity of RNA was determined using a Agilent Bioanalyzer as per manufacturers procedures (Agilent Technologies, Palo Alto, CA).
|
Label |
DIG-UTP
|
Label protocol |
1 ug of total RNA was used to transcribe DIG-labeled cRNA using Applied Biosystems Chemiluminescent RT-IVT Kit V2.0.
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|
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Hybridization protocol |
Microarray hybridization (using twenty micrograms of fragmented, DIG-labeled cRNA) and processing were performed according to Applied Biosystems protocols.
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Scan protocol |
Chemiluminescence detection, imaging, auto gridding, and image analysis was done according to Applied Biosystems protocols and the 1700 Chemiluminescent Microarray Analyzer Software v. 1.0.3.
|
Description |
Pooled lung tissue from tree wild type FVB/N mice treated with tumor inducible MCA/BHT
|
Data processing |
Signal intensities across microarrays were normalized using the quantile-quantile method (www.bioconductor.org).
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|
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Submission date |
Jan 04, 2007 |
Last update date |
Mar 22, 2007 |
Contact name |
Tanya Kalin |
Organization name |
University of Chicago
|
Department |
Medicine
|
Lab |
Pulmonary and Critical Care Medicine
|
Street address |
5841 S. Maryland Ave.
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60637 |
Country |
USA |
|
|
Platform ID |
GPL2995 |
Series (1) |
GSE6641 |
Foxm1 Transcription Factor Accelerates Formation of Lung Tumors |
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