|
| Status |
Public on Sep 28, 2007 |
| Title |
Lungs from Rosa26-Foxm1 mice 12 weeks after MCA/BHT treatment |
| Sample type |
RNA |
| |
|
| Source name |
left lung lobes from three Rosa26-Foxm1 mice treated with MCA/BHT
|
| Organism |
Mus musculus |
| Characteristics |
pooled left lung lobes from three Rosa26-Foxm1 mice 12 weeks after MCA/BHT treatment
|
| Treatment protocol |
Rosa26-Foxm1 mice were injected intraperitoneally (i.p.) with one dose of 3-methylcholanthrene (MCA; 15 µg/g mouse weight), followed by six weekly i.p. injections of butylated hydroxytoluene (BHT; the first dose was 150 µg/g mouse weight, and subsequent doses were 200 µg/g mouse weight)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNA STAT-60 Reagent (TEL-TEST, INC., Friendswood, TX) with modifications to remove DNA using the Qiagen RNAeasy columns and DNaseI Kit (Qiagen, Valencia, CA). RNA was stored at –70C in RNase-free H2O. Quality and quantity of RNA was determined using a Agilent Bioanalyzer as per manufacturers procedures (Agilent Technologies, Palo Alto, CA).
|
| Label |
DIG-UTP
|
| Label protocol |
1 ug of total RNA was used to transcribe DIG-labeled cRNA using Applied Biosystems Chemiluminescent RT-IVT Kit V2.0.
|
| |
|
| Hybridization protocol |
Microarray hybridization (using twenty micrograms of fragmented, DIG-labeled cRNA) and processing were performed according to Applied Biosystems protocols.
|
| Scan protocol |
Chemiluminescence detection, imaging, auto gridding, and image analysis was done according to Applied Biosystems protocols and the 1700 Chemiluminescent Microarray Analyzer Software v. 1.0.3.
|
| Description |
Pooled lung tissue from three Rosa26-Foxm1 mice treated with tumor inducible MCA/BHT
|
| Data processing |
Signal intensities across microarrays were normalized using the quantile-quantile method (www.bioconductor.org).
|
| |
|
| Submission date |
Jan 04, 2007 |
| Last update date |
Mar 22, 2007 |
| Contact name |
Tanya Kalin |
| Organization name |
University of Chicago
|
| Department |
Medicine
|
| Lab |
Pulmonary and Critical Care Medicine
|
| Street address |
5841 S. Maryland Ave.
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE6641 |
Foxm1 Transcription Factor Accelerates Formation of Lung Tumors |
|
