|
| Status |
Public on Mar 24, 2008 |
| Title |
3T3L1_adipocyte_TetOff_2 |
| Sample type |
RNA |
| |
|
| Source name |
RNA was collected from 3T3L1 adipocytes expression only Tet-off plasmid.
|
| Organism |
Mus musculus |
| Characteristics |
3T3L1 adipocytes with control plasmid.
3T3L1 adipocytes expressing Uncoupling protein 1 (UCP1).
|
| Biomaterial provider |
Jayaraman Laboratory
|
| Growth protocol |
3T3-L1 preadipocytes were grown until confluence and differentiated into adipocytes using a standard adipogenic cocktail.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Adipocytes (10 days after differentiation) were used for extracting total RNA using the Nucleospin II RNA isolation kit (Clontech).
|
| Label |
biotin
|
| Label protocol |
Codelink Expression assay protocol.
|
| |
|
| Hybridization protocol |
Codelink Expression assay protocol.
|
| Scan protocol |
Codelink Expression assay protocol.
|
| Description |
RNA quality was assessed using the Agilent 2100 Bioanalyzer.
|
| Data processing |
CodeLink Expression Analysis 4.1.0.29054
|
| |
|
| Submission date |
Jan 04, 2007 |
| Last update date |
Mar 24, 2008 |
| Contact name |
Arul Jayaraman |
| Organization name |
Texas A&M University
|
| Department |
Artie McFerrin Department of Chemical Engineering
|
| Street address |
3122 TAMU
|
| City |
College Station |
| State/province |
TX |
| ZIP/Postal code |
77843-3122 |
| Country |
USA |
| |
|
| Platform ID |
GPL2897 |
| Series (1) |
| GSE6643 |
Effect of forced uncoupling protein-1 expression on 3T3-L1 adipocyte gene expression |
|
