|
| Status |
Public on Jun 24, 2007 |
| Title |
BG03 CM p35 |
| Sample type |
RNA |
| |
|
| Source name |
Human embryonic stem cell line BG03, 35 passages in MEF-CM
|
| Organism |
Homo sapiens |
| Characteristics |
BG03 cell line, karyotypically normal, female
|
| Biomaterial provider |
Thomas Schulz, Novocell Inc.
|
| Treatment protocol |
No Treatment
|
| Growth protocol |
BG03 cells were maintained in standard MEF-CM conditions for 35 passages. See Xu et all Nat Biotechnol 19, 971-974 (2001).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent (Invitrogen, Inc.)
|
| Label |
Biotin-Labeled cRNA
|
| Label protocol |
Illlumina RNA amplification kit, according to the manufacturer’s protocol
|
| |
|
| Hybridization protocol |
Refer to the Illumina Gene Expression System Manual
|
| Scan protocol |
Refer to the Illumina Gene Expression System Manual
|
| Description |
Comparison of gene expression in human embyronic stem cells grown in MEF-CM or defined media conditions
|
| Data processing |
Raw data for each sample was output using the Illumina BeadStudio software
|
| |
|
| Submission date |
Jan 04, 2007 |
| Last update date |
Jan 08, 2007 |
| Contact name |
Tom Schulz |
| E-mail(s) |
tschulz@novocell.com
|
| Organization name |
Novocell
|
| Street address |
111 Riverbend Rd
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602 |
| Country |
USA |
| |
|
| Platform ID |
GPL2507 |
| Series (1) |
| GSE6645 |
Comparison of gene expression in human embyronic stem cells grown in MEF-CM or defined media conditions |
|
