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Sample GSM1540963 Query DataSets for GSM1540963
Status Public on Mar 31, 2015
Title SPL-Nai
Sample type SAGE
Anchor NlaIII
Tag Count 60000
Tag Length 10
 
Source name Splenic Thy1.1+ CD8+ T cells, from uninfected mice
Organism Mus musculus
Characteristics cell type: Splenic Thy1.1+ CD8+ T cells, from uninfected mice
strain: C57BL/6
Treatment protocol CD8+ Thy1.1+ T cells were sorted from uninfected mice
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with an RNeasy RNA isolation kit from Qiagen and processed as in the supplied protocol (see also Shires et al (2001), Immunity, 15, 419-34
Complete SAGE protocol provided in Series supplementary file Hayday_SAGE_Protocol_Nov 2001.pdf
 
Description tag count is approximate
Data processing supplementary files include data normalized to 100,000 tags
Analysis was performed using SAGE300 and eSAGE software
 
Submission date Nov 07, 2014
Last update date Mar 31, 2015
Contact name Lucie Abeler-Dörner
Organization name King's College London
Department Immunobiology
Lab Hayday
Street address Great Maze Pond
City London
ZIP/Postal code SE1 9RT
Country United Kingdom
 
Platform ID GPL11
Series (1)
GSE63090 Gene expression of conventional and unconventional T cell subsets

Supplementary file Size Download File type/resource
GSM1540963_Hayday_SAGE_library_3.txt.gz 33.0 Kb (ftp)(http) TXT
Processed data provided as supplementary file

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