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Status |
Public on Mar 31, 2015 |
Title |
SPL-Nai |
Sample type |
SAGE |
Anchor |
NlaIII
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Tag Count |
60000 |
Tag Length |
10 |
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Source name |
Splenic Thy1.1+ CD8+ T cells, from uninfected mice
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Organism |
Mus musculus |
Characteristics |
cell type: Splenic Thy1.1+ CD8+ T cells, from uninfected mice strain: C57BL/6
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Treatment protocol |
CD8+ Thy1.1+ T cells were sorted from uninfected mice
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated with an RNeasy RNA isolation kit from Qiagen and processed as in the supplied protocol (see also Shires et al (2001), Immunity, 15, 419-34 Complete SAGE protocol provided in Series supplementary file Hayday_SAGE_Protocol_Nov 2001.pdf
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Description |
tag count is approximate
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Data processing |
supplementary files include data normalized to 100,000 tags Analysis was performed using SAGE300 and eSAGE software
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Submission date |
Nov 07, 2014 |
Last update date |
Mar 31, 2015 |
Contact name |
Lucie Abeler-Dörner |
Organization name |
King's College London
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Department |
Immunobiology
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Lab |
Hayday
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Street address |
Great Maze Pond
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City |
London |
ZIP/Postal code |
SE1 9RT |
Country |
United Kingdom |
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Platform ID |
GPL11 |
Series (1) |
GSE63090 |
Gene expression of conventional and unconventional T cell subsets |
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Supplementary file |
Size |
Download |
File type/resource |
GSM1540963_Hayday_SAGE_library_3.txt.gz |
33.0 Kb |
(ftp)(http) |
TXT |
Processed data provided as supplementary file |
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