|
| Status |
Public on Dec 01, 2007 |
| Title |
AM14 B cells, anti IgM treated, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Murine AM-14 B cells
|
| Organism |
Mus musculus |
| Characteristics |
Splenic B cells from MRL/+ AM14 mice
|
| Treatment protocol |
F(ab?)2 goat anti-mouse IgM (Jackson ImmunoResearch Laboratories)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard Qiagen RNeasy mini kit protocol (www.qiagen.com)
|
| Label |
Biotin
|
| Label protocol |
Standard Ambion aRNA Message Amp protocols (www.ambion.com)
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol (www.affymetrix.com)
|
| Scan protocol |
Standard Affymetrix protocol (www.affymetrix.com)
|
| Description |
AM14 B cells treated with anti-IgM
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings in Genedata Expressionist and global scaling with mask correction as normalization method. The mean target intensity of each array was arbitrarily set to 1500.
|
| |
|
| Submission date |
Jan 08, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jason W Bauer |
| E-mail(s) |
baue0271@umn.edu
|
| Phone |
612-625-5168
|
| Organization name |
University of Minnesota
|
| Street address |
420 Deleware St SE MMC108
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6674 |
Gene expression program of AM-14 B cells stimulated through the B cell receptor (BCR) and/or Toll-like receptors (TLR) |
|
| Relations |
| Reanalyzed by |
GSE119085 |
