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Status |
Public on Mar 04, 2016 |
Title |
Activated T normal rep2 |
Sample type |
RNA |
|
|
Source name |
in vitro activated T cells from 5-week-old p53 KO mouse
|
Organism |
Mus musculus |
Characteristics |
strain background: C57Bl/6 genotype/variation: p53 KO age: 5 weeks tissue/cell type: in vitro activated, normal T cells
|
Treatment protocol |
Lymphoma cells or normal cells as control were purified by cell sorting from liver or spleen.
|
Growth protocol |
p53 deficient mice injected every 2 weeks with PBS or Heat-killer Streptococcus pneumoniae until disease development
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA were extract with with the Qiagen RNeasy minikit (Qiagen), then 50 ng of total RNA were amplified using GeneChip 3' IVT Express Kit from Affymetrix.
|
Label |
biotin
|
Label protocol |
As per manufacturer (Affymetrix)
|
|
|
Hybridization protocol |
As per manufacturer (Affymetrix)
|
Scan protocol |
The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0
|
Description |
SAMPLE 17
|
Data processing |
CHP files were processed using MAS 5.0 analysis algorithm with Affymetrix Expression Console software affymetrix-algorithm-name = ExpressionStat affymetrix-algorithm-version = 5.0 program-name = Expression Console program-id = 1.2.1.20
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|
|
Submission date |
Nov 13, 2014 |
Last update date |
Mar 05, 2016 |
Contact name |
GENESTIER Laurent |
E-mail(s) |
laurent.genestier@inserm.fr
|
Organization name |
INSERM
|
Street address |
Faculté de Medecine Lyon Sud. 165 Chemin du petit Revoyet
|
City |
Oullins |
ZIP/Postal code |
69921 |
Country |
France |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE63264 |
Expression of peripheral T lymphoma cells from p53 deficient |
GSE174257 |
Expression data from resting and activated murine T cell as well as murine peripheral T cell lymphomas |
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