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Sample GSM1544670 Query DataSets for GSM1544670
Status Public on Mar 04, 2016
Title Activated T normal rep2
Sample type RNA
 
Source name in vitro activated T cells from 5-week-old p53 KO mouse
Organism Mus musculus
Characteristics strain background: C57Bl/6
genotype/variation: p53 KO
age: 5 weeks
tissue/cell type: in vitro activated, normal T cells
Treatment protocol Lymphoma cells or normal cells as control were purified by cell sorting from liver or spleen.
Growth protocol p53 deficient mice injected every 2 weeks with PBS or Heat-killer Streptococcus pneumoniae until disease development
Extracted molecule total RNA
Extraction protocol Total RNA were extract with with the Qiagen RNeasy minikit (Qiagen), then 50 ng of total RNA were amplified using GeneChip 3' IVT Express Kit from Affymetrix.
Label biotin
Label protocol As per manufacturer (Affymetrix)
 
Hybridization protocol As per manufacturer (Affymetrix)
Scan protocol The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0
Description SAMPLE 17
Data processing CHP files were processed using MAS 5.0 analysis algorithm with Affymetrix Expression Console software
affymetrix-algorithm-name = ExpressionStat
affymetrix-algorithm-version = 5.0
program-name = Expression Console
program-id = 1.2.1.20
 
Submission date Nov 13, 2014
Last update date Mar 05, 2016
Contact name GENESTIER Laurent
E-mail(s) laurent.genestier@inserm.fr
Organization name INSERM
Street address Faculté de Medecine Lyon Sud. 165 Chemin du petit Revoyet
City Oullins
ZIP/Postal code 69921
Country France
 
Platform ID GPL1261
Series (2)
GSE63264 Expression of peripheral T lymphoma cells from p53 deficient
GSE174257 Expression data from resting and activated murine T cell as well as murine peripheral T cell lymphomas

Data table header descriptions
ID_REF
VALUE MAS5
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1310.49 P 0.00010954
AFFX-BioB-M_at 2031.09 P 4.42873e-05
AFFX-BioB-3_at 1483.2 P 0.000662269
AFFX-BioC-5_at 2817.68 P 9.4506e-05
AFFX-BioC-3_at 4618.79 P 5.16732e-05
AFFX-BioDn-5_at 6575.23 P 4.42873e-05
AFFX-BioDn-3_at 9550.5 P 0.000169227
AFFX-CreX-5_at 40622.4 P 5.16732e-05
AFFX-CreX-3_at 38894.4 P 4.42873e-05
AFFX-DapX-5_at 1258.31 P 6.02111e-05
AFFX-DapX-M_at 2185.85 P 9.4506e-05
AFFX-DapX-3_at 5443.14 P 4.42873e-05
AFFX-LysX-5_at 80.7308 P 0.0044838
AFFX-LysX-M_at 206.967 P 0.00687065
AFFX-LysX-3_at 659.26 P 0.000146402
AFFX-PheX-5_at 302.732 P 0.000445901
AFFX-PheX-M_at 514.87 P 4.42873e-05
AFFX-PheX-3_at 860.186 P 0.00010954
AFFX-ThrX-5_at 275.531 P 0.000169227
AFFX-ThrX-M_at 698.021 P 0.000195116

Total number of rows: 45101

Table truncated, full table size 1376 Kbytes.




Supplementary file Size Download File type/resource
GSM1544670_17_p53_19_Mouse430_2_.CEL.gz 3.4 Mb (ftp)(http) CEL
GSM1544670_17_p53_19_Mouse430_2_.mas5.CHP.gz 400.2 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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