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Sample GSM154501 Query DataSets for GSM154501
Status Public on May 18, 2007
Title GFP+ CD4+ cells from Foxp3(gfpko/wt) mice
Sample type RNA
 
Source name pooled lymph node and spleen
Organism Mus musculus
Characteristics Strain: C57BL/6 backcross 5 from 129/Sv
Gender: Female Age: 6-8 weeks
Treatment protocol Cells were isolated by magnetic bead sorting followed by fluorescence activated cell sorting.
Extracted molecule total RNA
Extraction protocol TelTest Stat-60 RNA extraction.
Label biotin
Label protocol RNA was converted to biotinylated cRNA (2 rounds of amplification) using commercially available reagent kits (MessageAmp II, Ambion, Austin, TX) and were hybridized to Affymetrix mouse 430 2.0 microarrays.
 
Hybridization protocol Standard hybridization to Affymetrix 430_2.0 arrays.
Scan protocol Standard scanning of Affymetrix 430_2.0 arrays.
Description Foxp3(gfpko/wt) hemizygous female mice express GFP in place of Foxp3 from the gfpko allele, allowing for the visualization of cells transcribing a mutant Foxp3 locus. Foxp3 is X-linked, thus cells have inactivated either the Foxp3(wt) or the Foxp3(gfpko) locus.
Data processing GCMRA, Bioconductor
 
Submission date Jan 09, 2007
Last update date Aug 28, 2018
Contact name Alexander Rudensky
Organization name University of Washington
Department Immunology
Street address 1959 Pacific St
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platform ID GPL1261
Series (2)
GSE7280 Gene expression in peripheral cells: effects of Foxp3 and PDE3B
GSE7773 Foxp3-dependent programme of regulatory T-cell differentiation
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Peripheral TFN cells

Data table
ID_REF VALUE
1415670_at 9.509520247
1415671_at 8.494795408
1415672_at 11.02565111
1415673_at 7.236714595
1415674_a_at 8.874571782
1415675_at 7.768536348
1415676_a_at 10.63517117
1415677_at 6.450925201
1415678_at 9.736162627
1415679_at 11.9580934
1415680_at 8.376398452
1415681_at 8.384260765
1415682_at 6.961983371
1415683_at 10.25706042
1415684_at 7.279861159
1415685_at 7.651632617
1415686_at 7.941532681
1415687_a_at 10.65170289
1415688_at 10.05179469
1415689_s_at 9.173164854

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM154501.CEL.gz 3.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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