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Sample GSM154502 Query DataSets for GSM154502
Status Public on May 18, 2007
Title GFP+ CD4+ cells from Foxp3(gfp/gfp) mice
Sample type RNA
Source name pooled lymph node and spleen
Organism Mus musculus
Characteristics Strain: C57BL/6 backcross 8-10 from 129/Sv
Gender: Female Age: 6-8 weeks
Treatment protocol Cells were isolated by magnetic bead sorting followed by fluorescence activated cell sorting.
Extracted molecule total RNA
Extraction protocol TelTest Stat-60 RNA extraction.
Label biotin
Label protocol RNA was converted to biotinylated cRNA (2 rounds of amplification) using commercially available reagent kits (MessageAmp II, Ambion, Austin, TX) and were hybridized to Affymetrix mouse 430 2.0 microarrays.
Hybridization protocol Standard hybridization to Affymetrix 430_2.0 arrays.
Scan protocol Standard scanning of Affymetrix 430_2.0 arrays.
Description Foxp3(gfp/gfp) homozygous female mice bear a GFP-Foxp3 fusion protein knockin allele, allowing for the visualization of regulatory T cells due to GFP-Foxp3 protein fluorescence.
Data processing GCMRA, Bioconductor
Submission date Jan 09, 2007
Last update date Aug 28, 2018
Contact name Alexander Rudensky
Organization name University of Washington
Department Immunology
Street address 1959 Pacific St
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
Platform ID GPL1261
Series (2)
GSE7280 Gene expression in peripheral cells: effects of Foxp3 and PDE3B
GSE7773 Foxp3-dependent programme of regulatory T-cell differentiation
Reanalyzed by GSE119085

Data table header descriptions
VALUE Peripheral TR cells

Data table
1415670_at 9.893257974
1415671_at 8.763729145
1415672_at 11.05003569
1415673_at 7.402391527
1415674_a_at 9.11865865
1415675_at 8.266463236
1415676_a_at 10.83939228
1415677_at 7.550311137
1415678_at 10.36046184
1415679_at 11.92574376
1415680_at 8.584114109
1415681_at 8.541674745
1415682_at 7.021222192
1415683_at 10.65388051
1415684_at 8.077347661
1415685_at 7.5011335
1415686_at 8.183897109
1415687_a_at 10.70207274
1415688_at 10.22472873
1415689_s_at 9.603593905

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM154502.CEL.gz 3.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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