|
| Status |
Public on Nov 11, 2015 |
| Title |
MLL5-overexpressing cells, biological rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
GBM primary culture G514NS_MLL5
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: glioblastoma (GBM) primary culture
genotype/variation: MLL5-overexpressing
time point: 72 h post-transfection
|
| Treatment protocol |
One million cells were transfected with plasmids for the expression of either EGFP or the N-terminal isoform of MLL5. Plasmid backbone was pcDNA-DEST40. Selection for transfected cells was performed with puromycin 24 h after transfection.
|
| Growth protocol |
Cells derived from an adult GBM patient and mantained in neural stem-like conditions according to a paper by Pollard et al (2009) Cell Stem Cell.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
72 h post-transfection, total RNA was extracted with an RNA extraction kit (RNeasy Mini Kit, QIAGEN).
|
| Label |
Biotin
|
| Label protocol |
Fragmented ss-cDNA was lebelled with biotin allonamide triphosphate.
|
| |
|
| Hybridization protocol |
Hybridization was performed at 45°C for 16 h.
|
| Scan protocol |
Scanning was performed with Affymetrix GeneChip Scanner 3000.
|
| Description |
HTA2_091814H_PD4_M1.CEL.pimg
Cells grown in NS media
|
| Data processing |
Normalization was performed with Partek Genomic Suite v. 6.6, applying RMA. All steps were performed according the Partek users manual.
the fold_change.txt contains p<0.05 >|1.2|fold data
|
| |
|
| Submission date |
Nov 14, 2014 |
| Last update date |
Dec 31, 2015 |
| Contact name |
Marco Gallo |
| E-mail(s) |
marco.gallo@ucalgary.ca
|
| Organization name |
University of Calgary
|
| Department |
Cumming School of Medicine
|
| Street address |
3330 Hospital Dr NW
|
| City |
Calgary |
| State/province |
AB |
| ZIP/Postal code |
T2N 4N1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE63296 |
MLL5 Orchestrates a Cancer Self-Renewal State by Repressing the Histone Variant H3.3 and Globally Reorganizing Chromatin [expression] |
|
