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Sample GSM154725 Query DataSets for GSM154725
Status Public on May 18, 2007
Title GFP+ CD4+ cells from Foxp3(gfp/gfp) mice treated with IL-2
Sample type RNA
 
Source name pooled lymph node and spleen
Organism Mus musculus
Characteristics Strain: C57BL/6 backcross 8-10 from 129/Sv
Gender: Female Age: 6-8 weeks
Treatment protocol Mice were injected with 50 ng/g IL-2 or vehicle (PBS) four times within 24 hrs. 30 min following the last treatment, cells were isolated by magnetic bead sorting followed by fluorescence activated cell sorting.
Extracted molecule total RNA
Extraction protocol TelTest Stat-60 RNA extraction.
Label biotin
Label protocol RNA was converted to biotinylated cRNA (2 rounds of amplification) using commercially available reagent kits (MessageAmp II, Ambion, Austin, TX) and were hybridized to Affymetrix mouse 430 2.0 microarrays.
 
Hybridization protocol Standard hybridization to Affymetrix 430_2.0 arrays.
Scan protocol Standard scanning of Affymetrix 430_2.0 arrays.
Description Foxp3(gfp/gfp) homozygous female mice bear a GFP-Foxp3 fusion protein knockin allele, allowing for the visualization of regulatory T cells due to GFP-Foxp3 protein fluorescence.
Data processing GCMRA, Bioconductor
 
Submission date Jan 10, 2007
Last update date Aug 28, 2018
Contact name Alexander Rudensky
Organization name University of Washington
Department Immunology
Street address 1959 Pacific St
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platform ID GPL1261
Series (2)
GSE7280 Gene expression in peripheral cells: effects of Foxp3 and PDE3B
GSE7773 Foxp3-dependent programme of regulatory T-cell differentiation
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Peripheral TR cells + IL2

Data table
ID_REF VALUE
1415670_at 9.79549802
1415671_at 8.722986465
1415672_at 11.14516617
1415673_at 7.672527652
1415674_a_at 9.095035219
1415675_at 8.514434435
1415676_a_at 10.72767592
1415677_at 7.9507183
1415678_at 10.41696993
1415679_at 11.76723545
1415680_at 8.675053056
1415681_at 8.559481402
1415682_at 7.228414708
1415683_at 10.74019134
1415684_at 8.11665128
1415685_at 7.572980948
1415686_at 7.996852113
1415687_a_at 10.8030111
1415688_at 10.11173576
1415689_s_at 9.422751995

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM154725.CEL.gz 3.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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