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Sample GSM154726 Query DataSets for GSM154726
Status Public on May 18, 2007
Title GFP- CD25+ CD4+ cells from Foxp3(gfp/gfp) mice treated with IL-2
Sample type RNA
 
Source name pooled lymph node and spleen
Organism Mus musculus
Characteristics Strain: C57BL/6 backcross 8-10 from 129/Sv Gender: Female Age: 6-8 weeks
Treatment protocol Mice were injected with 50 ng/g IL-2 or vehicle (PBS) four times within 24 hrs. 30 min following the last treatment, cells were isolated by magnetic bead sorting followed by fluorescence activated cell sorting.
Extracted molecule total RNA
Extraction protocol TelTest Stat-60 RNA extraction.
Label biotin
Label protocol RNA was converted to biotinylated cRNA (2 rounds of amplification) using commercially available reagent kits (MessageAmp II, Ambion, Austin, TX) and were hybridized to Affymetrix mouse 430 2.0 microarrays.
 
Hybridization protocol Standard hybridization to Affymetrix 430_2.0 arrays.
Scan protocol Standard scanning of Affymetrix 430_2.0 arrays.
Description Foxp3(gfp/gfp) homozygous female mice bear a GFP-Foxp3 fusion protein knockin allele, allowing for the visualization of regulatory T cells due to GFP-Foxp3 protein fluorescence.
Data processing GCMRA, Bioconductor
 
Submission date Jan 10, 2007
Last update date Aug 28, 2018
Contact name Alexander Rudensky
Organization name University of Washington
Department Immunology
Street address 1959 Pacific St
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platform ID GPL1261
Series (2)
GSE7280 Gene expression in peripheral cells: effects of Foxp3 and PDE3B
GSE7773 Foxp3-dependent programme of regulatory T-cell differentiation
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Peripheral T25 cells + IL2

Data table
ID_REF VALUE
1415670_at 9.683647232
1415671_at 8.812340747
1415672_at 11.3667129
1415673_at 7.571653778
1415674_a_at 9.054691539
1415675_at 8.044428758
1415676_a_at 10.80987403
1415677_at 6.814260154
1415678_at 10.12208518
1415679_at 11.75095776
1415680_at 8.757520355
1415681_at 8.415098558
1415682_at 7.354913195
1415683_at 10.73064365
1415684_at 7.779178096
1415685_at 7.654106211
1415686_at 8.019309477
1415687_a_at 10.75092723
1415688_at 10.2764022
1415689_s_at 8.92346239

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM154726.CEL.gz 3.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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