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Sample GSM154727 Query DataSets for GSM154727
Status Public on May 18, 2007
Title GFP+ CD4+ cells from Foxp3(gfpko/wt) mice treated with IL-2
Sample type RNA
 
Source name pooled lymph node and spleen
Organism Mus musculus
Characteristics Strain: C57BL/6 backcross 5 from 129/Sv
Gender: Female Age: 6-8 weeks
Treatment protocol Mice were injected with 50 ng/g IL-2 or vehicle (PBS) four times within 24 hrs. 30 min following the last treatment, cells were isolated by magnetic bead sorting followed by fluorescence activated cell sorting.
Extracted molecule total RNA
Extraction protocol TelTest Stat-60 RNA extraction.
Label biotin
Label protocol RNA was converted to biotinylated cRNA (2 rounds of amplification) using commercially available reagent kits (MessageAmp II, Ambion, Austin, TX) and were hybridized to Affymetrix mouse 430 2.0 microarrays.
 
Hybridization protocol Standard hybridization to Affymetrix 430_2.0 arrays.
Scan protocol Standard scanning of Affymetrix 430_2.0 arrays.
Description Foxp3(gfpko/wt) hemizygous female mice express GFP in place of Foxp3 from the gfpko allele, allowing for the visualization of cells transcribing a mutant Foxp3 locus. Foxp3 is X-linked, thus cells have inactivated either the Foxp3(wt) or the Foxp3(gfpko) locus.
Data processing GCMRA, Bioconductor
 
Submission date Jan 10, 2007
Last update date Aug 28, 2018
Contact name Alexander Rudensky
Organization name University of Washington
Department Immunology
Street address 1959 Pacific St
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platform ID GPL1261
Series (2)
GSE7280 Gene expression in peripheral cells: effects of Foxp3 and PDE3B
GSE7773 Foxp3-dependent programme of regulatory T-cell differentiation
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Peripheral TFN cells + IL2

Data table
ID_REF VALUE
1415670_at 9.779635831
1415671_at 8.643208428
1415672_at 11.38406823
1415673_at 7.793219827
1415674_a_at 9.35012898
1415675_at 8.273158681
1415676_a_at 10.95156521
1415677_at 7.061185905
1415678_at 9.959293057
1415679_at 11.95068764
1415680_at 8.45109467
1415681_at 8.945729567
1415682_at 7.06634421
1415683_at 10.50941965
1415684_at 7.787900927
1415685_at 7.874718562
1415686_at 7.89322644
1415687_a_at 10.8980553
1415688_at 9.931220705
1415689_s_at 9.253274556

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM154727.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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