|
| Status |
Public on May 18, 2007 |
| Title |
GFP+ CD4+ cells from Foxp3(gfpko/wt) mice treated with IL-2 |
| Sample type |
RNA |
| |
|
| Source name |
pooled lymph node and spleen
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6 backcross 5 from 129/Sv
Gender: Female Age: 6-8 weeks
|
| Treatment protocol |
Mice were injected with 50 ng/g IL-2 or vehicle (PBS) four times within 24 hrs. 30 min following the last treatment, cells were isolated by magnetic bead sorting followed by fluorescence activated cell sorting.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TelTest Stat-60 RNA extraction.
|
| Label |
biotin
|
| Label protocol |
RNA was converted to biotinylated cRNA (2 rounds of amplification) using commercially available reagent kits (MessageAmp II, Ambion, Austin, TX) and were hybridized to Affymetrix mouse 430 2.0 microarrays.
|
| |
|
| Hybridization protocol |
Standard hybridization to Affymetrix 430_2.0 arrays.
|
| Scan protocol |
Standard scanning of Affymetrix 430_2.0 arrays.
|
| Description |
Foxp3(gfpko/wt) hemizygous female mice express GFP in place of Foxp3 from the gfpko allele, allowing for the visualization of cells transcribing a mutant Foxp3 locus. Foxp3 is X-linked, thus cells have inactivated either the Foxp3(wt) or the Foxp3(gfpko) locus.
|
| Data processing |
GCMRA, Bioconductor
|
| |
|
| Submission date |
Jan 10, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Alexander Rudensky |
| Organization name |
University of Washington
|
| Department |
Immunology
|
| Street address |
1959 Pacific St
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE7280 |
Gene expression in peripheral cells: effects of Foxp3 and PDE3B |
| GSE7773 |
Foxp3-dependent programme of regulatory T-cell differentiation |
|
| Relations |
| Reanalyzed by |
GSE119085 |
