|
| Status |
Public on Mar 31, 2015 |
| Title |
BTN_B3 |
| Sample type |
RNA |
| |
|
| Source name |
MODE-K cells, transduced with Btnl1, TNF+IL-1_, 18h
|
| Organism |
Mus musculus |
| Characteristics |
strain: C3H/He
genotype: female
cell line: SV40 T transformed intestinal epithelial cell line
retroviral vector: pMX-Btnl1-IRES-GFP
|
| Treatment protocol |
MODE-K cells were stimulated with unstimulated or stimulated (on anti-CD3) cultured IEL supernatant or a combination of TNF (1 µg/ml) and IL-1_ (100 pg/ml) for 18 hours.
|
| Growth protocol |
MODE-K cells stably transfected with Btnl1 or vector alone were seeded in 6-well plates the day before the experiment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a Qiagen RNEasy extraction kit.
|
| Label |
biotin
|
| Label protocol |
Standard Illumina biotin labelling protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
5511176031_A
|
| Data processing |
Quantile normalization with no Background subtraction using Genome Studio.
|
| |
|
| Submission date |
Nov 17, 2014 |
| Last update date |
Mar 31, 2015 |
| Contact name |
Lucie Abeler-Dörner |
| Organization name |
King's College London
|
| Department |
Immunobiology
|
| Lab |
Hayday
|
| Street address |
Great Maze Pond
|
| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE63364 |
In vitro activation of Btnl-1 transduced MODE-K cells with different stimuli |
|
