NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1547511 Query DataSets for GSM1547511
Status Public on Mar 31, 2015
Title GFP_B2
Sample type RNA
 
Source name MODE-K cells, transduced with empty vector (GFP), TNF+IL-1_, 18h
Organism Mus musculus
Characteristics strain: C3H/He
genotype: female
cell line: SV40 T transformed intestinal epithelial cell line
retroviral vector: pMX-IRES-GFP
Treatment protocol MODE-K cells were stimulated with unstimulated or stimulated (on anti-CD3) cultured IEL supernatant or a combination of TNF (1 µg/ml) and IL-1_ (100 pg/ml) for 18 hours.
Growth protocol MODE-K cells stably transfected with Btnl1 or vector alone were seeded in 6-well plates the day before the experiment.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using a Qiagen RNEasy extraction kit.
Label biotin
Label protocol Standard Illumina biotin labelling protocol
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 2
5511176052_G
Data processing Quantile normalization with no Background subtraction using Genome Studio.
 
Submission date Nov 17, 2014
Last update date Mar 31, 2015
Contact name Lucie Abeler-Dörner
Organization name King's College London
Department Immunobiology
Lab Hayday
Street address Great Maze Pond
City London
ZIP/Postal code SE1 9RT
Country United Kingdom
 
Platform ID GPL6885
Series (1)
GSE63364 In vitro activation of Btnl-1 transduced MODE-K cells with different stimuli

Data table header descriptions
ID_REF
VALUE Quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 7.198374317 0.1817043
ILMN_1212612 7.601311727 0.005012531
ILMN_1212619 9.050481563 0
ILMN_1212628 7.125763705 0.2781955
ILMN_1212632 7.554109153 0.0112782
ILMN_1212636 13.38293198 0
ILMN_1212637 10.2051811 0
ILMN_1212645 6.970212902 0.6867168
ILMN_1212648 9.008273139 0
ILMN_1212653 8.922797558 0
ILMN_1212672 9.122207567 0
ILMN_1212682 7.283562535 0.0877193
ILMN_1212683 6.74775125 0.9799499
ILMN_1212685 7.109562197 0.3082707
ILMN_1212692 9.462787512 0
ILMN_1212693 8.626004291 0
ILMN_1212695 7.029058587 0.5137845
ILMN_1212698 6.870179747 0.8847118
ILMN_1212702 7.32151326 0.06390978
ILMN_1212703 7.682611218 0

Total number of rows: 25697

Table truncated, full table size 804 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap