|
| Status |
Public on Dec 01, 2015 |
| Title |
HL53-2 Muscle |
| Sample type |
RNA |
| |
|
| Source name |
Muscle tissue
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Muscle
genotype/variation: p53 flow/flox
|
| Treatment protocol |
No treatment was used
|
| Growth protocol |
Transgenic mice bearing p53 mutation were monitored until tumors were readily observable and/or limb paralysis symptoms appeared
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using standard Trizol method
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
fresh frozen
Replicate 1
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Nov 25, 2014 |
| Last update date |
Dec 01, 2015 |
| Contact name |
Yuning Jackie Tang |
| Organization name |
Hospital for Sick Children
|
| Department |
Program in Developmental Biology
|
| Street address |
686 Bay Street
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE63631 |
Transcriptome analysis of a novel mouse model of osteosarcoma and soft tissue sarcoma |
|
