|
Status |
Public on Dec 19, 2008 |
Title |
CD4+CD25+ Treg, B6.H2g7, rep3 |
Sample type |
RNA |
|
|
Source name |
CD4+CD25+ Treg cells from B6.H2g7 mouse spleens
|
Organism |
Mus musculus |
Characteristics |
Genetic background: B6 congenic for MHC Class II Ag7 (H2g7) Age: 6 wks Number of mice per chip: 3 Tissue: Spleen Sorting: MACS for CD25+ cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25hi cells %Foxp3+ after restaining: 96%
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
|
Label |
Biotin
|
Label protocol |
BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
|
|
|
Hybridization protocol |
Per Joslin Diabetes Center microarray core facility.
|
Scan protocol |
Per Joslin Diabetes Center microarray core facility.
|
Description |
CEL File: jos3503.CEL
|
Data processing |
RMA algorithm, through GenePattern module ExpressionFileCreator from the Broad Institute.
|
|
|
Submission date |
Jan 17, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
CBDM Lab |
E-mail(s) |
cbdm@hms.harvard.edu
|
Phone |
617-432-7747
|
Organization name |
Harvard Medical School
|
Department |
Microbiology and Immunobiology
|
Lab |
CBDM
|
Street address |
77 Avenue Louis Pasteur
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02215 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE6813 |
Gene expression profiles of CD4+CD25+ Tregs from NOD and B6.H2g7 mice |
|
Relations |
Reanalyzed by |
GSE119085 |