|
| Status |
Public on Dec 19, 2008 |
| Title |
CD4+CD25+ Treg, NOD, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
CD4+CD25+ Treg cells from NOD mouse spleens
|
| Organism |
Mus musculus |
| Characteristics |
Genetic background: NOD
Age: 6 wks
Number of mice per chip: 3
Tissue: Spleen
Sorting: MACS for CD25+ cells, then double-sorted for CD8a-CD11b-CD69-B220- CD4+CD25hi cells
%Foxp3+ after restaining: 95%
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
|
| Label |
Biotin
|
| Label protocol |
BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
|
| |
|
| Hybridization protocol |
Per Joslin Diabetes Center microarray core facility.
|
| Scan protocol |
Per Joslin Diabetes Center microarray core facility.
|
| Description |
CEL File: jos3493.CEL
|
| Data processing |
RMA algorithm, through GenePattern module ExpressionFileCreator from the Broad Institute.
|
| |
|
| Submission date |
Jan 17, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6813 |
Gene expression profiles of CD4+CD25+ Tregs from NOD and B6.H2g7 mice |
|
| Relations |
| Reanalyzed by |
GSE119085 |
