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Sample GSM155729 Query DataSets for GSM155729
Status Public on Oct 20, 2007
Title Muscle control 7
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
Label Biotin
Label protocol One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Vastus lateralis muscle
Data processing The R statistical software was applied for data analysis. The RMA method was used for global background correction and qspline was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test was used to calculate p-values.
 
Submission date Jan 17, 2007
Last update date Feb 02, 2007
Contact name Vibe Skov
E-mail(s) vihs@regionsjaelland.dk
Phone +4526178735
Organization name Roskilde Hospital
Department Hematology
Street address Koegevej 7-13
City Roskilde
ZIP/Postal code 4000
Country Denmark
 
Platform ID GPL570
Series (1)
GSE6798 Reduced expression of mitochondrial oxidative metabolism genes in skeletal muscle of women with PCOS

Data table header descriptions
ID_REF
VALUE Normalised expression value

Data table
ID_REF VALUE
1007_s_at 370.7896466
1053_at 68.89893892
117_at 144.1817615
121_at 416.7784957
1255_g_at 25.58550268
1294_at 144.2127331
1316_at 391.3812662
1320_at 67.70560246
1405_i_at 125.811907
1431_at 13.40484426
1438_at 114.3589267
1487_at 453.9812236
1494_f_at 280.2445288
1552256_a_at 123.604481
1552257_a_at 134.0801221
1552258_at 54.38973568
1552261_at 427.608324
1552263_at 35.09397818
1552264_a_at 234.2348427
1552266_at 25.07569

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM155729.cel.gz 8.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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