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Sample GSM156170 Query DataSets for GSM156170
Status Public on Oct 20, 2007
Title Muscle control 8
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
Label Biotin
Label protocol One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Vastus lateralis muscle
Data processing The R statistical software was applied for data analysis. The RMA method was used for global background correction and qspline was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test was used to calculate p-values.
 
Submission date Jan 17, 2007
Last update date Feb 02, 2007
Contact name Vibe Skov
E-mail(s) vihs@regionsjaelland.dk
Phone +4526178735
Organization name Roskilde Hospital
Department Hematology
Street address Koegevej 7-13
City Roskilde
ZIP/Postal code 4000
Country Denmark
 
Platform ID GPL570
Series (1)
GSE6798 Reduced expression of mitochondrial oxidative metabolism genes in skeletal muscle of women with PCOS

Data table header descriptions
ID_REF
VALUE Normalised expression value

Data table
ID_REF VALUE
1007_s_at 440.0230499
1053_at 88.38687758
117_at 134.0503943
121_at 395.8661877
1255_g_at 25.08734195
1294_at 191.4586506
1316_at 567.0378975
1320_at 89.66311985
1405_i_at 77.82858096
1431_at 19.2489868
1438_at 119.7592361
1487_at 427.6689306
1494_f_at 223.8136424
1552256_a_at 152.9023542
1552257_a_at 126.6214448
1552258_at 47.13102359
1552261_at 282.4865844
1552263_at 32.20021319
1552264_a_at 289.1792728
1552266_at 25.77331253

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM156170.cel.gz 8.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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