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Sample GSM156176 Query DataSets for GSM156176
Status Public on Oct 20, 2007
Title Muscle control 10
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
Label Biotin
Label protocol One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Vastus lateralis muscle
Data processing The R statistical software was applied for data analysis. The RMA method was used for global background correction and qspline was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test was used to calculate p-values.
 
Submission date Jan 17, 2007
Last update date Feb 02, 2007
Contact name Vibe Skov
E-mail(s) vihs@regionsjaelland.dk
Phone +4526178735
Organization name Roskilde Hospital
Department Hematology
Street address Koegevej 7-13
City Roskilde
ZIP/Postal code 4000
Country Denmark
 
Platform ID GPL570
Series (1)
GSE6798 Reduced expression of mitochondrial oxidative metabolism genes in skeletal muscle of women with PCOS

Data table header descriptions
ID_REF
VALUE Normalised expression value

Data table
ID_REF VALUE
1007_s_at 522.4234324
1053_at 64.85922387
117_at 157.9322494
121_at 470.6809985
1255_g_at 35.21351685
1294_at 187.1373627
1316_at 592.7897041
1320_at 80.25415558
1405_i_at 72.1613621
1431_at 25.35795345
1438_at 113.8330642
1487_at 479.6158453
1494_f_at 282.228683
1552256_a_at 177.3039165
1552257_a_at 130.0284305
1552258_at 58.72542426
1552261_at 319.0490032
1552263_at 37.8914527
1552264_a_at 246.2057076
1552266_at 13.56990239

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM156176.cel.gz 8.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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