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Sample GSM156177 Query DataSets for GSM156177
Status Public on Oct 20, 2007
Title Muscle control 11
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
Label Biotin
Label protocol One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Vastus lateralis muscle
Data processing The R statistical software was applied for data analysis. The RMA method was used for global background correction and qspline was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test was used to calculate p-values.
 
Submission date Jan 17, 2007
Last update date Feb 02, 2007
Contact name Vibe Skov
E-mail(s) vihs@regionsjaelland.dk
Phone +4526178735
Organization name Roskilde Hospital
Department Hematology
Street address Koegevej 7-13
City Roskilde
ZIP/Postal code 4000
Country Denmark
 
Platform ID GPL570
Series (1)
GSE6798 Reduced expression of mitochondrial oxidative metabolism genes in skeletal muscle of women with PCOS

Data table header descriptions
ID_REF
VALUE Normalised expression value

Data table
ID_REF VALUE
1007_s_at 463.9729176
1053_at 41.72599688
117_at 184.0227885
121_at 459.1827206
1255_g_at 18.23603869
1294_at 144.5472247
1316_at 482.3202883
1320_at 66.86753981
1405_i_at 39.45030309
1431_at 17.15755233
1438_at 87.47846721
1487_at 512.7207771
1494_f_at 185.012865
1552256_a_at 169.3775515
1552257_a_at 110.6624473
1552258_at 29.97037844
1552261_at 169.2959735
1552263_at 32.77545991
1552264_a_at 199.2139749
1552266_at 14.76425407

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM156177.cel.gz 7.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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