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Sample GSM156187 Query DataSets for GSM156187
Status Public on Oct 20, 2007
Title Muscle PCOS 3
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
Label Biotin
Label protocol One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Vastus lateralis muscle
Data processing The R statistical software was applied for data analysis. The RMA method was used for global background correction and qspline was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test was used to calculate p-values.
 
Submission date Jan 17, 2007
Last update date Feb 02, 2007
Contact name Vibe Skov
E-mail(s) vihs@regionsjaelland.dk
Phone +4526178735
Organization name Roskilde Hospital
Department Hematology
Street address Koegevej 7-13
City Roskilde
ZIP/Postal code 4000
Country Denmark
 
Platform ID GPL570
Series (1)
GSE6798 Reduced expression of mitochondrial oxidative metabolism genes in skeletal muscle of women with PCOS

Data table header descriptions
ID_REF
VALUE Normalised expression value

Data table
ID_REF VALUE
1007_s_at 514.9162957
1053_at 72.42117114
117_at 145.6526063
121_at 399.2077394
1255_g_at 22.52492713
1294_at 207.3222499
1316_at 597.4437186
1320_at 82.03281766
1405_i_at 41.13366651
1431_at 26.24538098
1438_at 122.2130556
1487_at 499.98941
1494_f_at 187.6296175
1552256_a_at 175.6067761
1552257_a_at 113.7046397
1552258_at 52.63383914
1552261_at 150.4822335
1552263_at 29.5257559
1552264_a_at 246.0180544
1552266_at 19.6469185

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM156187.cel.gz 8.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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