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Sample GSM156512 Query DataSets for GSM156512
Status Public on Oct 20, 2007
Title Muscle PCOS 9
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
Label Biotin
Label protocol One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Vastus lateralis muscle
Data processing The R statistical software was applied for data analysis. The RMA method was used for global background correction and qspline was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test was used to calculate p-values.
 
Submission date Jan 17, 2007
Last update date Aug 28, 2018
Contact name Vibe Skov
E-mail(s) vihs@regionsjaelland.dk
Phone +4526178735
Organization name Roskilde Hospital
Department Hematology
Street address Koegevej 7-13
City Roskilde
ZIP/Postal code 4000
Country Denmark
 
Platform ID GPL570
Series (1)
GSE6798 Reduced expression of mitochondrial oxidative metabolism genes in skeletal muscle of women with PCOS
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Normalised expression value

Data table
ID_REF VALUE
1007_s_at 492.8606028
1053_at 56.33792359
117_at 175.3733166
121_at 476.6688391
1255_g_at 27.63199031
1294_at 146.271969
1316_at 613.1586092
1320_at 77.36498802
1405_i_at 49.8396549
1431_at 18.15212203
1438_at 82.42397487
1487_at 441.2512409
1494_f_at 188.6786021
1552256_a_at 133.5361613
1552257_a_at 124.5798565
1552258_at 40.76169546
1552261_at 236.0427458
1552263_at 23.89025612
1552264_a_at 263.9580856
1552266_at 10.63116573

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM156512.cel.gz 8.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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