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Sample GSM1566659 Query DataSets for GSM1566659
Status Public on Nov 25, 2021
Title wildtype control biological replicate 1
Sample type RNA
 
Source name wildtype mouse, control diet
Organism Mus musculus
Characteristics genotype/variation: wildtype
strain: C57BL/6
gender: male
diet: control
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Affymetrix Mouse Gene 1.0 ST array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
Data processing The data were analyzed with Affymetrix Exression Console version 1.4 using RMA
 
Submission date Dec 16, 2014
Last update date Nov 25, 2021
Contact name Jamie Barger
E-mail(s) jamie.l.barger@gmail.com
Organization name LifeGen Technologies, LLC
Street address 510 Charmany Drive, Suite 263
City Madison
State/province WI
ZIP/Postal code 53719
Country USA
 
Platform ID GPL6246
Series (1)
GSE64229 Gene expression in gastrocnemius muscle in response to calorie restriction in wildtype and Sirt3 knockout mice

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
10338001 12.0925
10338002 6.01368
10338003 10.5738
10338004 9.43191
10338005 1.64248
10338006 1.85651
10338007 2.31567
10338008 3.49755
10338009 7.7002
10338010 1.67783
10338011 5.43541
10338012 1.75638
10338013 1.5464
10338014 1.58848
10338015 1.53943
10338016 7.11553
10338017 12.7338
10338018 6.39871
10338019 4.83865
10338020 7.53346

Total number of rows: 35556

Table truncated, full table size 586 Kbytes.




Supplementary file Size Download File type/resource
GSM1566659_wildtype-co06.CEL.gz 3.8 Mb (ftp)(http) CEL
GSM1566659_wildtype-co06.rma-gene-default.chp.gz 269.4 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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