|
| Status |
Public on Nov 25, 2021 |
| Title |
sirt3ko_cr biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Sirt3 knockout mouse, calorie restricted diet
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Sirt3 knockout
strain: C57BL/6
gender: male
diet: calorie restricted
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Affymetrix Mouse Gene 1.0 ST array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
|
| Data processing |
The data were analyzed with Affymetrix Exression Console version 1.4 using RMA
|
| |
|
| Submission date |
Dec 16, 2014 |
| Last update date |
Nov 25, 2021 |
| Contact name |
Jamie Barger |
| E-mail(s) |
jamie.l.barger@gmail.com
|
| Organization name |
LifeGen Technologies, LLC
|
| Street address |
510 Charmany Drive, Suite 263
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53719 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE64229 |
Gene expression in gastrocnemius muscle in response to calorie restriction in wildtype and Sirt3 knockout mice |
|
