|
| Status |
Public on Oct 20, 2007 |
| Title |
Muscle PCOS 12 |
| Sample type |
RNA |
| |
|
| Source name |
Vastus lateralis muscle
|
| Organism |
Homo sapiens |
| Characteristics |
Vastus lateralis muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
|
| Label |
Biotin
|
| Label protocol |
One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Vastus lateralis muscle
|
| Data processing |
The R statistical software was applied for data analysis. The RMA method was used for global background correction and qspline was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test was used to calculate p-values.
|
| |
|
| Submission date |
Jan 18, 2007 |
| Last update date |
Feb 02, 2007 |
| Contact name |
Vibe Skov |
| E-mail(s) |
vihs@regionsjaelland.dk
|
| Phone |
+4526178735
|
| Organization name |
Roskilde Hospital
|
| Department |
Hematology
|
| Street address |
Koegevej 7-13
|
| City |
Roskilde |
| ZIP/Postal code |
4000 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE6798 |
Reduced expression of mitochondrial oxidative metabolism genes in skeletal muscle of women with PCOS |
|
