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Sample GSM156753 Query DataSets for GSM156753
Status Public on Oct 20, 2007
Title Muscle PCOS 14
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
Label Biotin
Label protocol One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Vastus lateralis muscle
Data processing The R statistical software was applied for data analysis. The RMA method was used for global background correction and qspline was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test was used to calculate p-values.
 
Submission date Jan 18, 2007
Last update date Aug 28, 2018
Contact name Vibe Skov
E-mail(s) vihs@regionsjaelland.dk
Phone +4526178735
Organization name Roskilde Hospital
Department Hematology
Street address Koegevej 7-13
City Roskilde
ZIP/Postal code 4000
Country Denmark
 
Platform ID GPL570
Series (1)
GSE6798 Reduced expression of mitochondrial oxidative metabolism genes in skeletal muscle of women with PCOS
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Normalised expression value

Data table
ID_REF VALUE
1007_s_at 448.5957895
1053_at 62.07775108
117_at 137.1133508
121_at 382.7697308
1255_g_at 30.1676013
1294_at 173.1024235
1316_at 439.6340139
1320_at 72.61273018
1405_i_at 86.62926996
1431_at 18.25744114
1438_at 108.192001
1487_at 522.7746727
1494_f_at 271.2026447
1552256_a_at 147.0499819
1552257_a_at 94.26580942
1552258_at 33.73458661
1552261_at 421.4276018
1552263_at 37.44216151
1552264_a_at 247.3005108
1552266_at 12.44674596

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM156753.cel.gz 7.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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