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Sample GSM156946 Query DataSets for GSM156946
Status Public on Oct 20, 2007
Title Muscle PCOS 19
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
Label Biotin
Label protocol One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Vastus lateralis muscle
Data processing The R statistical software was applied for data analysis. The RMA method was used for global background correction and qspline was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test was used to calculate p-values.
 
Submission date Jan 18, 2007
Last update date Aug 28, 2018
Contact name Vibe Skov
E-mail(s) vihs@regionsjaelland.dk
Phone +4526178735
Organization name Roskilde Hospital
Department Hematology
Street address Koegevej 7-13
City Roskilde
ZIP/Postal code 4000
Country Denmark
 
Platform ID GPL570
Series (1)
GSE6798 Reduced expression of mitochondrial oxidative metabolism genes in skeletal muscle of women with PCOS
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Normalised expression value

Data table
ID_REF VALUE
1007_s_at 494.7331844
1053_at 69.8442683
117_at 123.0320817
121_at 388.8304603
1255_g_at 24.2783921
1294_at 160.4891419
1316_at 481.0701643
1320_at 63.9323554
1405_i_at 78.8633042
1431_at 21.94469021
1438_at 90.40965656
1487_at 442.8360401
1494_f_at 185.1167541
1552256_a_at 171.6151331
1552257_a_at 98.49971609
1552258_at 30.19906897
1552261_at 230.6083504
1552263_at 32.64493608
1552264_a_at 242.8039525
1552266_at 19.10049696

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM156946.cel.gz 8.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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