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Sample GSM157255 Query DataSets for GSM157255
Status Public on Dec 31, 2007
Title Ovarian tumor AM225
Sample type RNA
 
Source name epithelian ovarian tumor
Organism Homo sapiens
Characteristics Tissue: epithelial ovarian tumor, Tumor type: serous, Tumor stage: benign
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 15 microg total RNA.
 
Hybridization protocol 15 ug of total RNA was reverse-transcribed with oligo-dT primer containing a T7 RNA polymerase-binding site. An in vitro transcription was performed on this cDNA. DNA from the samples was fragmented in 40 mM Tris acetate, 100 mM potassium acetate, and 30 mM MgCl2 (pH 8.1) at 95°C to reduce secondary structures. 15 ug of cRNA were hybridized to an Affymetrix HuGeneFL microarray, washed and stained.
Scan protocol Scanned with a Hewlett Packard Gene Array Scanner
Description low malignant potential
Data processing Raw values assigned by MAS 4
 
Submission date Jan 22, 2007
Last update date Feb 25, 2008
Contact name Jaroslav Petr Novak
E-mail(s) jaroslav.novak@mail.mcgill.ca, jaroslav.novak@gmail.com
Phone 450-465-1383
Fax 450-465-3033
Organization name McGill University and Genome Quebec Innovation Centre
Department Microarray Platform
Street address 740, Dr Penfield Avenue
City Montreal
State/province Quebec
ZIP/Postal code H3A 1A4
Country Canada
 
Platform ID GPL80
Series (1)
GSE6822 Classification of ovarian tumor samples

Data table header descriptions
ID_REF
VALUE MAS4-calculated Signal intensity
ABS_CALL Affymetrix call, present (P), absent (A) or marginal (M)

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at -14.1 A
AFFX-BioB-M_at -30.2 A
AFFX-BioB-3_at 1.8 A
AFFX-BioC-5_at 20.9 A
AFFX-BioC-3_at -28.4 A
AFFX-BioDn-5_at -103 A
AFFX-BioDn-3_at 41.2 A
AFFX-CreX-5_at -16.2 A
AFFX-CreX-3_at -6.2 A
AFFX-BioB-5_st 224.7 A
AFFX-BioB-M_st 20.4 A
AFFX-BioB-3_st -103.5 A
AFFX-BioC-5_st -12.8 A
AFFX-BioC-3_st -22.6 A
AFFX-BioDn-5_st 81.9 A
AFFX-BioDn-3_st 28.5 A
AFFX-CreX-5_st -13.8 A
AFFX-CreX-3_st -6.5 A
hum_alu_at 18059.3 P
AFFX-DapX-5_at 0.7 A

Total number of rows: 7129

Table truncated, full table size 128 Kbytes.




Supplementary file Size Download File type/resource
GSM157255.CEL.gz 2.0 Mb (ftp)(http) CEL

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