NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM157675 Query DataSets for GSM157675
Status Public on Jan 30, 2007
Title unstimulated embryonic fibroblasts WT, biological rep1
Sample type RNA
 
Source name embryonic fibroblast basal transcription
Organism Mus musculus
Characteristics Genotype: wild type BL/6
Primary cell extract
Treatment protocol Fibroblasts were left untreated and RNA was extracted using Trizol solution (GibcoBRL).
Growth protocol Cells were grown in DMEM (GibcoBRL) supplemented with 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Hybridization Oven 640.
Scan protocol GeneChips were scanned using the GeneChip Array Scanner 7G
Description Gene expression data from unstimulated EF cells.
Data processing the data were analyzed with GeneChip Operating Software (GCOS) ver 1.3.
 
Submission date Jan 22, 2007
Last update date Aug 28, 2018
Contact name Benjamin Robert tenOever
Organization name Harvard University
Department MCB
Lab Maniatis
Street address 7 Divinity Avenue
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL1261
Series (1)
GSE6837 Expression data from wild type (wt) and Ikbke knockout (Ikke) embryonic fibroblasts (EF)
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity

Data table
ID_REF VALUE
1415670_at 5604
1415671_at 1933.2
1415672_at 4354.6
1415673_at 1482.2
1415674_a_at 2768.2
1415675_at 993.4
1415676_a_at 6438.1
1415677_at 753.4
1415678_at 2535
1415679_at 2211.4
1415680_at 4488.2
1415681_at 4657
1415682_at 1048.7
1415683_at 4799.2
1415684_at 547.4
1415685_at 918.1
1415686_at 1997.7
1415687_a_at 6617
1415688_at 2709.3
1415689_s_at 425.2

Total number of rows: 45101

Table truncated, full table size 731 Kbytes.




Supplementary file Size Download File type/resource
GSM157675.CEL.gz 5.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap