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Sample GSM157677 Query DataSets for GSM157677
Status Public on Jan 30, 2007
Title unstimulated embryonic fibroblasts WT, biological rep3
Sample type RNA
 
Source name embryonic fibroblast basal transcription
Organism Mus musculus
Characteristics Genotype: wild type BL/6
Primary cell extract
Treatment protocol Fibroblasts were left untreated and RNA was extracted using Trizol solution (GibcoBRL).
Growth protocol Cells were grown in DMEM (GibcoBRL) supplemented with 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Hybridization Oven 640.
Scan protocol GeneChips were scanned using the GeneChip Array Scanner 7G
Description Gene expression data from unstimulated EF cells.
Data processing the data were analyzed with GeneChip Operating Software (GCOS) ver 1.3.
 
Submission date Jan 22, 2007
Last update date Jan 30, 2007
Contact name Benjamin Robert tenOever
Organization name Harvard University
Department MCB
Lab Maniatis
Street address 7 Divinity Avenue
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL339
Series (1)
GSE6837 Expression data from wild type (wt) and Ikbke knockout (Ikke) embryonic fibroblasts (EF)

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity

Data table
ID_REF VALUE
1415670_at 2938.4
1415671_at 2557.2
1415672_at 1621.6
1415673_at 584.9
1415674_a_at 755.5
1415675_at 952.6
1415676_a_at 4389.4
1415677_at 262.6
1415678_at 1100.7
1415679_at 2184.8
1415680_at 988.6
1415681_at 752.9
1415682_at 409.6
1415683_at 3252.8
1415684_at 121
1415685_at 341.5
1415686_at 629.5
1415687_a_at 5656.7
1415688_at 1187.2
1415689_s_at 365.4

Total number of rows: 22690

Table truncated, full table size 380 Kbytes.




Supplementary file Size Download File type/resource
GSM157677.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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