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Sample GSM157679 Query DataSets for GSM157679
Status Public on Jan 30, 2007
Title unstimulated embryonic fibroblasts KO, biological rep2
Sample type RNA
 
Source name embryonic fibroblast basal transcription
Organism Mus musculus
Characteristics Genotype: ikbke -/- BL/6
Primary cell extract
Treatment protocol Fibroblasts were left untreated and RNA was extracted using Trizol solution (GibcoBRL).
Growth protocol Cells were grown in DMEM (GibcoBRL) supplemented with 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Hybridization Oven 640.
Scan protocol GeneChips were scanned using the GeneChip Array Scanner 7G
Description Gene expression data from unstimulated EF cells.
Data processing the data were analyzed with GeneChip Operating Software (GCOS) ver 1.3.
 
Submission date Jan 22, 2007
Last update date Jan 30, 2007
Contact name Benjamin Robert tenOever
Organization name Harvard University
Department MCB
Lab Maniatis
Street address 7 Divinity Avenue
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL339
Series (1)
GSE6837 Expression data from wild type (wt) and Ikbke knockout (Ikke) embryonic fibroblasts (EF)

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity

Data table
ID_REF VALUE
1415670_at 2848.1
1415671_at 2541.2
1415672_at 1700.4
1415673_at 640.1
1415674_a_at 1052.6
1415675_at 847
1415676_a_at 4685
1415677_at 235
1415678_at 1500.3
1415679_at 2114.7
1415680_at 1400.3
1415681_at 1021.5
1415682_at 482.6
1415683_at 3796.8
1415684_at 200.1
1415685_at 476
1415686_at 572.1
1415687_a_at 5918.8
1415688_at 1301.7
1415689_s_at 421.4

Total number of rows: 22690

Table truncated, full table size 379 Kbytes.




Supplementary file Size Download File type/resource
GSM157679.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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