|
| Status |
Public on Jan 31, 2007 |
| Title |
unstimulated embryonic fibroblasts KO, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
embryonic fibroblast basal transcription
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: ikbke -/- BL/6
Primary cell extract
|
| Treatment protocol |
Fibroblasts were left untreated and RNA was extracted using Trizol solution (GibcoBRL).
|
| Growth protocol |
Cells were grown in DMEM (GibcoBRL) supplemented with 10% Fetal Bovine Serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Hybridization Oven 640.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Array Scanner 7G
|
| Description |
Gene expression data from unstimulated EF cells.
|
| Data processing |
the data were analyzed with GeneChip Operating Software (GCOS) ver 1.3.
|
| |
|
| Submission date |
Jan 22, 2007 |
| Last update date |
Jan 31, 2007 |
| Contact name |
Benjamin Robert tenOever |
| Organization name |
Harvard University
|
| Department |
MCB
|
| Lab |
Maniatis
|
| Street address |
7 Divinity Avenue
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE6837 |
Expression data from wild type (wt) and Ikbke knockout (Ikke) embryonic fibroblasts (EF) |
|
