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Sample GSM157680 Query DataSets for GSM157680
Status Public on Jan 31, 2007
Title unstimulated embryonic fibroblasts KO, biological rep3
Sample type RNA
 
Source name embryonic fibroblast basal transcription
Organism Mus musculus
Characteristics Genotype: ikbke -/- BL/6
Primary cell extract
Treatment protocol Fibroblasts were left untreated and RNA was extracted using Trizol solution (GibcoBRL).
Growth protocol Cells were grown in DMEM (GibcoBRL) supplemented with 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Hybridization Oven 640.
Scan protocol GeneChips were scanned using the GeneChip Array Scanner 7G
Description Gene expression data from unstimulated EF cells.
Data processing the data were analyzed with GeneChip Operating Software (GCOS) ver 1.3.
 
Submission date Jan 22, 2007
Last update date Jan 31, 2007
Contact name Benjamin Robert tenOever
Organization name Harvard University
Department MCB
Lab Maniatis
Street address 7 Divinity Avenue
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL339
Series (1)
GSE6837 Expression data from wild type (wt) and Ikbke knockout (Ikke) embryonic fibroblasts (EF)

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity

Data table
ID_REF VALUE
1415670_at 2692.5
1415671_at 2294.6
1415672_at 2071.8
1415673_at 749.3
1415674_a_at 1142.1
1415675_at 666.8
1415676_a_at 4356.9
1415677_at 389.3
1415678_at 1632
1415679_at 2061.4
1415680_at 1018.9
1415681_at 1004.6
1415682_at 510.3
1415683_at 3299.5
1415684_at 300.2
1415685_at 476.8
1415686_at 807.2
1415687_a_at 4313
1415688_at 1730.2
1415689_s_at 500.5

Total number of rows: 22690

Table truncated, full table size 379 Kbytes.




Supplementary file Size Download File type/resource
GSM157680.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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