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Sample GSM157685 Query DataSets for GSM157685
Status Public on Jan 31, 2007
Title stimulated embryonic fibroblasts KO, biological rep2
Sample type RNA
 
Source name embryonic fibroblast transcription profile 6 hours post interferon treatment
Organism Mus musculus
Characteristics Genotype: Ikbke-/- BL/6
Primary cell extract
Treatment protocol Fibroblasts were treated with interferon and RNA was extracted using Trizol solution (GibcoBRL).
Growth protocol Cells were grown in DMEM (GibcoBRL) supplemented with 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Hybridization Oven 640.
Scan protocol GeneChips were scanned using the GeneChip Array Scanner 7G
Description Gene expression data from unstimulated EF cells.
Data processing the data were analyzed with GeneChip Operating Software (GCOS) ver 1.3.
 
Submission date Jan 22, 2007
Last update date Aug 28, 2018
Contact name Benjamin Robert tenOever
Organization name Harvard University
Department MCB
Lab Maniatis
Street address 7 Divinity Avenue
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL1261
Series (1)
GSE6837 Expression data from wild type (wt) and Ikbke knockout (Ikke) embryonic fibroblasts (EF)
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity

Data table
ID_REF VALUE
1415670_at 2989
1415671_at 1351.8
1415672_at 3868.4
1415673_at 2595.8
1415674_a_at 2530.6
1415675_at 996.7
1415676_a_at 7831.2
1415677_at 753.8
1415678_at 2158
1415679_at 2386.6
1415680_at 4494.5
1415681_at 3130.8
1415682_at 1164.8
1415683_at 5034.2
1415684_at 591.1
1415685_at 1052.3
1415686_at 1549
1415687_a_at 4469.2
1415688_at 3127.5
1415689_s_at 369.8

Total number of rows: 45101

Table truncated, full table size 732 Kbytes.




Supplementary file Size Download File type/resource
GSM157685.CEL.gz 5.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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