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Sample GSM157686 Query DataSets for GSM157686
Status Public on Jan 31, 2007
Title stimulated embryonic fibroblasts KO, biological rep3
Sample type RNA
 
Source name embryonic fibroblast transcription profile 6 hours post interferon treatment
Organism Mus musculus
Characteristics Genotype: Ikbke-/- BL/6
Primary cell extract
Treatment protocol Fibroblasts were treated with interferon and RNA was extracted using Trizol solution (GibcoBRL).
Growth protocol Cells were grown in DMEM (GibcoBRL) supplemented with 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Hybridization Oven 640.
Scan protocol GeneChips were scanned using the GeneChip Array Scanner 7G
Description Gene expression data from unstimulated EF cells.
Data processing the data were analyzed with GeneChip Operating Software (GCOS) ver 1.3.
 
Submission date Jan 22, 2007
Last update date Aug 28, 2018
Contact name Benjamin Robert tenOever
Organization name Harvard University
Department MCB
Lab Maniatis
Street address 7 Divinity Avenue
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL1261
Series (1)
GSE6837 Expression data from wild type (wt) and Ikbke knockout (Ikke) embryonic fibroblasts (EF)
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity

Data table
ID_REF VALUE
1415670_at 1357.7
1415671_at 3571.2
1415672_at 3799.2
1415673_at 449.6
1415674_a_at 1650.1
1415675_at 1020.9
1415676_a_at 3240.9
1415677_at 904.5
1415678_at 2662.1
1415679_at 4898.1
1415680_at 1344.7
1415681_at 1481
1415682_at 555.3
1415683_at 2410.2
1415684_at 716
1415685_at 755.2
1415686_at 2368.9
1415687_a_at 13716.2
1415688_at 2332.8
1415689_s_at 1224.9

Total number of rows: 45101

Table truncated, full table size 740 Kbytes.




Supplementary file Size Download File type/resource
GSM157686.CEL.gz 6.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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